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Mouse Monoclonal Brn-2 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human POU3F2 aa 1-100.


Images

Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [CL6228] (AB243045), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (AB243045), expandable thumbnail
  • Western blot - Anti-Brn-2 antibody [CL6228] (AB243045), expandable thumbnail
  • Western blot - Anti-Brn-2 antibody [CL6228] (AB243045), expandable thumbnail
  • Western blot - Anti-Brn-2 antibody [CL6228] (AB243045), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human POU3F2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P20265

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Expected
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Predicted
Predicted
Predicted

Tested
Tested

Species
Mouse
Dilution info
1/200.00000 - 1/500.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
2.00000-10.00000 µg/mL
Notes

Fixation/Permeabilization: PFA/Triton X-100.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1 µg/mL
Notes

-

Species
Human
Dilution info
1 µg/mL
Notes

-

Predicted
Predicted

Species
Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).

Alternative names

BRN2, OCT7, OTF7, POU3F2, Brain-specific homeobox/POU domain protein 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Octamer-binding transcription factor 7, Brain-2, Brn-2, Oct-7, OTF-7

Recommended products

Mouse Monoclonal Brn-2 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human POU3F2 aa 1-100.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human POU3F2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link P20265
Clone number
CL6228
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Brn-2 also known as POU domain class 3 transcription factor 2 (POU3F2) is a transcription factor with a molecular weight of approximately 48 kDa. This protein plays a significant role in the development and function of the central nervous system. It is abundantly expressed in neuronal tissues and specific types of melanocytes. Brn-2 belongs to the POU domain family of proteins which are pivotal in regulating gene expression during development.

Biological function summary

Brn-2 regulates the expression of genes critical for neural differentiation and development. It acts as a transcriptional activator that binds to specific DNA sequences. Brn-2 does not act alone; it often participates in regulatory complexes with other transcription factors. Through these interactions Brn-2 influences the fate of neural progenitor cells and is involved in the regulation of neural stem cell maintenance.

Pathways

Brn-2 plays a role in the Wnt signaling pathway and the Notch signaling pathway. Both of these pathways are integral to cell fate determination during development. In the Wnt pathway Brn-2 may interact with proteins such as β-catenin which modulates the expression of genes involved in cell proliferation and differentiation. Additionally Brn-2's role in the Notch pathway can influence the activity of proteins like RBPJ impacting cell communication and developmental processes.

Associated diseases and disorders

Brn-2 has been linked to melanoma and gliomas. In melanoma Brn-2 can interact with other proteins such as MITF influencing melanocyte development and contributing to cancer progression. In glioma Brn-2's aberrant expression might affect cellular pathways that lead to uncontrolled proliferation and tumor formation. Understanding these connections provides insights into potential therapeutic targets for these conditions.

Product promise

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Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [CL6228] (ab243045), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [CL6228] (ab243045)

    PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Brn-2 (green) using ab243045 at 10 μg/ml in ICC/IF. Microtubules are stained red.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045)

    Paraffin-embedded mouse forebrain tissue stained for Brn-2 with ab243045 at 1/200 dilution in immunohistochemical analysis.

  • Western blot - Anti-Brn-2 antibody [CL6228] (ab243045), expandable thumbnail

    Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)

    Remaining relative intensity is presented.

    Loading control: Anti-GAPDH

    All lanes: Western blot - Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/mL

    Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) cells transfected with control siRNA

    Lane 2: HE-K293 cells transfected with target specific siRNA probe #1

    Predicted band size: 46 kDa

  • Western blot - Anti-Brn-2 antibody [CL6228] (ab243045), expandable thumbnail

    Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)

    All lanes: Western blot - Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/mL

    All lanes: U-251 MG (human brain glioma cell line) cell lysate

    Predicted band size: 46 kDa

  • Western blot - Anti-Brn-2 antibody [CL6228] (ab243045), expandable thumbnail

    Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)

    All lanes: Western blot - Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/mL

    All lanes: Mouse cerebral cortex tissue lysate

    Predicted band size: 46 kDa

  • Western blot - Anti-Brn-2 antibody [CL6228] (ab243045), expandable thumbnail

    Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)

    All lanes: Western blot - Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/mL

    All lanes: Human cerebral cortex tissue lysate

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045)

    Paraffin-embedded mouse cerebral cortex tissue stained for Brn-2 with ab243045 at 1/200 dilution in immunohistochemical analysis. Reactivity observed in layer 2-4 neurones.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (ab243045)

    Paraffin-embedded mouse cerebral cortex tissue stained for Brn-2 with ab243045 at 1/200 dilution in immunohistochemical analysis. No reactivity in layer 6 neurones.

  • Western blot - Anti-Brn-2 antibody [CL6228] (ab243045), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Brn-2 antibody [CL6228] (ab243045)

    Brn-2 western blot using anti-Brn-2 antibody [CL6228] ab243045. Publication image and figure legend from Ding, S., Jin, Y., et al., 2020, Cancer Cell Int, PubMed 31920461.


    ab243045 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab243045 please see the product overview.

    Associations among BCYRN1, miR-490-3p, and POU3F2 were assessed by western blot and qRT-PCR. The mRNA a and protein b expression of POU3F2 in HepG2 cells were evaluated after knockdown of BCYRN1, miR-490-3p, or BCYRN1 + miR-490-3p. c is statistical of b. **P < 0.01 vs. control, #P < 0.01 vs. miR-490-3p inhibitor. The mRNA d and protein e expression of POU3F2 in BEL-7402 cells were determined after overexpression of BCYRN1, miR-490-3p, or BCYRN1 + miR-490-3p. f is statistical of e. **P < 0.01 vs. control, ##P < 0.01 vs. miR-490-3p mimic

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