AB243045

Anti-Brn-2 antibody [CL6228]

Name: Anti-Brn-2 antibody [CL6228] (ab243045) | Abcam
Brand: Abcam Limited
SKU: AB243045
Price: 630 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(4 Publications)

Mouse Monoclonal Brn-2 antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human POU3F2 aa 1-100.

View Alternative Names

BRN2, OCT7, OTF7, POU3F2, Brain-specific homeobox/POU domain protein 2, Nervous system-specific octamer-binding transcription factor N-Oct-3, Octamer-binding protein 7, Octamer-binding transcription factor 7, Brain-2, Brn-2, Oct-7, OTF-7

9 Images

Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [CL6228] (AB243045)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [CL6228] (AB243045)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for Brn-2 (green) using ab243045 at 10 μg/ml in ICC/IF. Microtubules are stained red.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (AB243045)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (AB243045)

Paraffin-embedded mouse cerebral cortex tissue stained for Brn-2 with ab243045 at 1/200 dilution in immunohistochemical analysis. Reactivity observed in layer 2-4 neurones.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (AB243045)

Paraffin-embedded mouse forebrain tissue stained for Brn-2 with ab243045 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (AB243045)

Paraffin-embedded mouse cerebral cortex tissue stained for Brn-2 with ab243045 at 1/200 dilution in immunohistochemical analysis. No reactivity in layer 6 neurones.

Supplier Data

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

All lanes:

Western blot - Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/mL

All lanes:

U-251 MG (human brain glioma cell line) cell lysate

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

All lanes:

Western blot - Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/mL

All lanes:

Mouse cerebral cortex tissue lysate

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

All lanes:

Western blot - Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/mL

All lanes:

Human cerebral cortex tissue lysate

Predicted band size: 46 kDa

false

Supplier Data

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

Remaining relative intensity is presented.

Loading control : Anti-GAPDH

All lanes:

Western blot - Anti-Brn-2 antibody [CL6228] (ab243045) at 1 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cells transfected with control siRNA

Lane 2:

HEK-293 cells transfected with target specific siRNA probe #1

Predicted band size: 46 kDa

false

CiteAb

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

Brn-2 western blot using anti-Brn-2 antibody [CL6228] ab243045. Publication image and figure legend from Ding, S., Jin, Y., et al., 2020, Cancer Cell Int, PubMed 31920461.

ab243045 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab243045 please see the product overview.

Associations among BCYRN1, miR-490-3p, and POU3F2 were assessed by western blot and qRT-PCR. The mRNA a and protein b expression of POU3F2 in HepG2 cells were evaluated after knockdown of BCYRN1, miR-490-3p, or BCYRN1 + miR-490-3p. c is statistical of b. **p < 0.01 vs. control, #p < 0.01 vs. miR-490-3p inhibitor. The mRNA d and protein e expression of POU3F2 in BEL-7402 cells were determined after overexpression of BCYRN1, miR-490-3p, or BCYRN1 + miR-490-3p. f is statistical of e. **p < 0.01 vs. control, ##p < 0.01 vs. miR-490-3p mimic

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL6228

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human POU3F2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

P20265

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Brn-2 also known as POU domain class 3 transcription factor 2 (POU3F2) is a transcription factor with a molecular weight of approximately 48 kDa. This protein plays a significant role in the development and function of the central nervous system. It is abundantly expressed in neuronal tissues and specific types of melanocytes. Brn-2 belongs to the POU domain family of proteins which are pivotal in regulating gene expression during development.

Biological function summary

Brn-2 regulates the expression of genes critical for neural differentiation and development. It acts as a transcriptional activator that binds to specific DNA sequences. Brn-2 does not act alone; it often participates in regulatory complexes with other transcription factors. Through these interactions Brn-2 influences the fate of neural progenitor cells and is involved in the regulation of neural stem cell maintenance.

Pathways

Brn-2 plays a role in the Wnt signaling pathway and the Notch signaling pathway. Both of these pathways are integral to cell fate determination during development. In the Wnt pathway Brn-2 may interact with proteins such as β-catenin which modulates the expression of genes involved in cell proliferation and differentiation. Additionally Brn-2's role in the Notch pathway can influence the activity of proteins like RBPJ impacting cell communication and developmental processes.

Brn-2 has been linked to melanoma and gliomas. In melanoma Brn-2 can interact with other proteins such as MITF influencing melanocyte development and contributing to cancer progression. In glioma Brn-2's aberrant expression might affect cellular pathways that lead to uncontrolled proliferation and tumor formation. Understanding these connections provides insights into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).

See full target information POU3F2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2105 PubMed40025041

2025

Disruption of tryptophan metabolism by high-fat diet-triggered maternal immune activation promotes social behavioral deficits in male mice.

Applications

Unspecified application

Species

Unspecified reactive species

Penghao Sun,Mengli Wang,Xuejun Chai,Yong-Xin Liu,Luqi Li,Wei Zheng,Shulin Chen,Xiaoyan Zhu,Shanting Zhao

Cell death and differentiation 32:447-465 PubMed39402212

2024

SOX4 facilitates brown fat development and maintenance through EBF2-mediated thermogenic gene program in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Wang,Ting He,Ya Luo,Kexin Ren,Huanming Shen,Lingfeng Hou,Yixin Wei,Tong Fu,Wenlong Xie,Peng Wang,Jie Hu,Yu Zhu,Zhengrong Huang,Qiyuan Li,Weihua Li,Huiling Guo,Boan Li

International journal of medical sciences 21:265-276 PubMed38169586

2024

LINC00662 promotes melanoma progression by competitively binding miR-107 and activating the β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Luo,Rui Lei,Qingfang Zhao,Yichen Shen,Zhigang He,Jinghong Xu

Cancer cell international 20:6 PubMed31920461

2020

LncRNA BCYRN1/miR-490-3p/POU3F2, served as a ceRNA network, is connected with worse survival rate of hepatocellular carcinoma patients and promotes tumor cell growth and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Shichao Ding,Yanfeng Jin,Qingzhi Hao,Yanmeng Kang,Ruiping Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Brn-2 antibody [CL6228]

Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [CL6228] (AB243045)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (AB243045)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (AB243045)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brn-2 antibody [CL6228] (AB243045)

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

Western blot - Anti-Brn-2 antibody [CL6228] (AB243045)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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