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Rabbit Multiclonal Brn-2 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human POU3F2.

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Images

Western blot - Anti-Brn-2 antibody [RP23040248] (AB308102), expandable thumbnail
  • Western blot - Anti-Brn-2 antibody [RP23040248] (AB308102), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [RP23040248] (AB308102), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human POU3F2. Database link P20265

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWB
Human
Tested
Tested
Mouse
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200

Notes

-

Species

Mouse

Dilution info

1/200

Notes

-

Target data

Function

Transcription factor that plays a key role in neuronal differentiation (By similarity). Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity). Acts as a transcriptional activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene promoters (By similarity). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (By similarity). Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (By similarity).

Alternative names

Recommended products

Rabbit Multiclonal Brn-2 antibody. Suitable for ICC/IF, WB and reacts with Human, Mouse samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human POU3F2.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human POU3F2. Database link P20265
Clone number

RP23040248

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Brn-2 also known as POU domain class 3 transcription factor 2 (POU3F2) is a transcription factor with a molecular weight of approximately 48 kDa. This protein plays a significant role in the development and function of the central nervous system. It is abundantly expressed in neuronal tissues and specific types of melanocytes. Brn-2 belongs to the POU domain family of proteins which are pivotal in regulating gene expression during development.

Biological function summary

Brn-2 regulates the expression of genes critical for neural differentiation and development. It acts as a transcriptional activator that binds to specific DNA sequences. Brn-2 does not act alone; it often participates in regulatory complexes with other transcription factors. Through these interactions Brn-2 influences the fate of neural progenitor cells and is involved in the regulation of neural stem cell maintenance.

Pathways

Brn-2 plays a role in the Wnt signaling pathway and the Notch signaling pathway. Both of these pathways are integral to cell fate determination during development. In the Wnt pathway Brn-2 may interact with proteins such as β-catenin which modulates the expression of genes involved in cell proliferation and differentiation. Additionally Brn-2's role in the Notch pathway can influence the activity of proteins like RBPJ impacting cell communication and developmental processes.

Associated diseases and disorders

Brn-2 has been linked to melanoma and gliomas. In melanoma Brn-2 can interact with other proteins such as MITF influencing melanocyte development and contributing to cancer progression. In glioma Brn-2's aberrant expression might affect cellular pathways that lead to uncontrolled proliferation and tumor formation. Understanding these connections provides insights into potential therapeutic targets for these conditions.

Product promise

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3 product images

  • Western blot - Anti-Brn-2 antibody [RP23040248] (ab308102), expandable thumbnail

    Western blot - Anti-Brn-2 antibody [RP23040248] (ab308102)

    Knockdown of Brn-2 was achieved by transfecting A-375 cells with Brn-2 specific siRNA. Western blot analysis was performed using modified whole cell extracts (1% SDS) from the A-375 knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blots were probed with ab308102 at 1/200 dilution and a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4,000 dilution. Loss of signal upon siRNA mediated knock down confirms that antibody is specific to POU3F2.

    All lanes: Western blot - Anti-Brn-2 antibody [RP23040248] (ab308102) at 1/200 dilution

    Lane 1: untransfected A375 cell lysate

    Lane 2: non-specific siRNA transfected A375 cell lysate

    Lane 3: Brn-2 specific siRNA transfected A375 cell lysate

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Observed band size: 54 kDa

  • Western blot - Anti-Brn-2 antibody [RP23040248] (ab308102), expandable thumbnail

    Western blot - Anti-Brn-2 antibody [RP23040248] (ab308102)

    Western blot analysis was performed on modified whole cell extracts (1%SDS) (30 µg lysate) of U-87 MG (Lane 1) and tissue extracts of Mouse pup brain (Lane 2) and Mouse brain (Lane 3). The blots were probed with ab308102 at 1/200 dilution and detected by chemiluminescence using a Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution. A ~54 kDa band corresponding to POU3F2 was observed across the cell lines and tissues tested.

    All lanes: Western blot - Anti-Brn-2 antibody [RP23040248] (ab308102) at 1/200 dilution

    Lane 1: U-87 MG cell lysate at 30 µg

    Lane 2: Mouse pup brain tissue lysate at 30 µg

    Lane 3: Mouse brain tissue lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

    Observed band size: 54 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [RP23040248] (ab308102), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody [RP23040248] (ab308102)

    For immunofluorescence analysis, Neural stem cells were fixed and permeabilized for detection of endogenous Brn-2 using ab308102 at 1/100 dilution and labeled with a Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution.

    Panel a) shows representative cells that were stained for detection and localization of Brn-2 protein (green)

    Panel b) is stained for nuclei (blue) using DAPI

    Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin at 1/300 dilution

    Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of Brn-2

    Panel e) represents control cells with no primary antibody to assess background

    The images were captured at 60X magnification

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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