Anti-BRN3A antibody [EP1972Y]

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BRN3A antibody [EP1972Y] (AB81213)

Overlay histogram showing SH-SY5Y cells stained withunpurified ab81213 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81213, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BRN3A antibody [EP1972Y] (AB81213)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling BRN3A with purified ab81213 at 1/100 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• WB

Lab

Western blot - Anti-BRN3A antibody [EP1972Y] (AB81213)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution

Lane 1:

SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Mouse cerebellum lysates at 20 µg

Lane 3:

Rat brain lysates at 20 µg

Lane 4:

Rat spinal cord lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

false

• WB

AbReview22876****

Western blot - Anti-BRN3A antibody [EP1972Y] (AB81213)

All lanes:

Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213) at 1/2000 dilution

Lane 1:

Adult mouse cerebellum lysate at 20 µg

Lane 2:

Adult mouse lower brainstem lysate at 20 µg

Secondary

All lanes:

HRP-conjugated Mouse anti-Rabbit IgG monoclonal. at 1/10000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

Exposure time: 2min

This image is courtesy of an Anonymous Abreview.

• WB

Unknown

Western blot - Anti-BRN3A antibody [EP1972Y] (AB81213)

All lanes:

Western blot - Anti-BRN3A antibody [EP1972Y] (ab81213)

Lane 1:

LNCaP

Lane 2:

SW480

Lane 3:

PC-3

Lane 4:

Human brain lysate

Predicted band size: 43 kDa

Observed band size: 43 kDa

false