Anti-BRN3B / POU4F2 antibody [EPR28922-88]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRN3B / POU4F2 antibody [EPR28922-88] (AB325130)

Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling BRN3B / POU4F2 with ab325130 at 1/100 (5.18 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human retina (PMID : 33380270).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRN3B / POU4F2 antibody [EPR28922-88] (AB325130)

Immunohistochemical analysis of paraffin-embedded Mouse retina tissue labeling BRN3B / POU4F2 with ab325130 at 1/100 (5.18 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse retina (PMID : 7691107).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRN3B / POU4F2 antibody [EPR28922-88] (AB325130)

Immunohistochemical analysis of paraffin-embedded Mouse E14.5 (Eyeball section) tissue labeling BRN3B / POU4F2 with ab325130 at 1/100 (5.18 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse E14.5 eyeball. (A) Low‑powered (magnification, x40) and (B) high‑powered (magnification, x400) microscopic images (PMID : 26962139).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRN3B / POU4F2 antibody [EPR28922-88] (AB325130)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling BRN3B / POU4F2 with ab325130 at 1/100 (5.18 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on mouse liver.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRN3B / POU4F2 antibody [EPR28922-88] (AB325130)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling BRN3B / POU4F2 with ab325130 at 1/100 (5.18 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human liver (PMID : 11470235).

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRN3B / POU4F2 antibody [EPR28922-88] (AB325130)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse POU4F2 expression vector containing a his tag, (B) HEK-293T transfected with a mouse POU4F1 expression vector containing a his tag, (C) HEK-293T transfected with a mouse POU4F3 expression vector containing a his tag and (D) HEK-293T cells transfected with empty vector containing a myc-His-tag labeling BRN3B / POU4F2 with ab325130 at 1/2000 (0.259 μg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining (A) HEK-293T transfected with a mouse POU4F2 expression vector containing a his tag, no staining on (B) HEK-293T transfected with a mouse POU4F1 expression vector containing a his tag, (C) HEK-293T transfected with a mouse POU4F3 expression vector containing a his tag and (D) HEK-293T cells transfected with empty vector containing a myc-His-tag.

The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• I-ELISA

Lab

Indirect ELISA - Anti-BRN3B / POU4F2 antibody [EPR28922-88] (AB325130)

Indirect ELISA analysis of ab325130 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human POU4F2.

Antigen concentration : 1000 ng/ml

• Dot

Lab

Dot Blot - Anti-BRN3B / POU4F2 antibody [EPR28922-88] (AB325130)

Dot blot analysis of BRN3B / POU4F2 using ab325130 at 1 : 1000 (0.518 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : 293T cells transfected with a human BRN3B / POU4F2 expression vector containing a myc-His-tag®, whole cell lysate

Lane 2 : 293T cells transfected with a human POU4F3 expression vector containing a myc-His-tag®, whole cell lysate

Lane 3 : 293T cells transfected with a human POU4F1 expression vector containing a myc-His-tag®, whole cell lysate

Lane 4 : 293T cells transfected with a mouse BRN3B / POU4F2 expression vector containing a myc-His-tag®, whole cell lysate

Lane 5 : 293T cells transfected with a mouse POU4F1 expression vector containing a myc-His-tag®, whole cell lysate

Lane 6 : 293T cells transfected with a mouse POU4F3 expression vector containing a myc-His-tag®, whole cell lysate

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with POU4F1 and POU4F3.

All lanes:

Dot Blot - Anti-BRN3B / POU4F2 antibody [EPR28922-88] (ab325130) at 1/1000 dilution

Lane 1:

293T cells transfected with a human BRN3B / POU4F2 expression vector containing a myc-His-tag®, whole cell lysate

Lane 2:

293T cells transfected with a human POU4F3 expression vector containing a myc-His-tag®, whole cell lysate

Lane 3:

293T cells transfected with a human POU4F1 expression vector containing a myc-His-tag®, whole cell lysate

Lane 4:

293T cells transfected with a mouse BRN3B / POU4F2 expression vector containing a myc-His-tag®, whole cell lysate

Lane 5:

293T cells transfected with a mouse POU4F1 expression vector containing a myc-His-tag®, whole cell lysate

Lane 6:

293T cells transfected with a mouse POU4F3 expression vector containing a myc-His-tag®, whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s