AB154105

Anti-BRRN1 antibody

Name: Anti-BRRN1 antibody (ab154105) | Abcam
Brand: Abcam Limited
SKU: AB154105
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal BRRN1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NCAPH aa 400 to C-terminus.

View Alternative Names

BRRN, BRRN1, CAPH, KIAA0074, NCAPH, Condensin complex subunit 2, Barren homolog protein 1, Chromosome-associated protein H, Non-SMC condensin I complex subunit H, XCAP-H homolog, hCAP-H

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-BRRN1 antibody (AB154105)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BRRN1 antibody (AB154105)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling BRRN1 with ab154105 at 1/200 dilution. Lower image show cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRRN1 antibody (AB154105)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRRN1 antibody (AB154105)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling BRRN1 with ab154105 at 1/500 dilution.

Unknown

Western blot - Anti-BRRN1 antibody (AB154105)

7.5% SDS PAGE

All lanes:

Western blot - Anti-BRRN1 antibody (ab154105) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 83 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NCAPH aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q15003

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases (PubMed : 11136719). Early in neurogenesis, may play an essential role to ensure accurate mitotic chromosome condensation in neuron stem cells, ultimately affecting neuron pool and cortex size (PubMed : 27737959).

See full target information NCAPH

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature 617:386-394 PubMed37100912

2023

A druggable copper-signalling pathway that drives inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Stéphanie Solier,Sebastian Müller,Tatiana Cañeque,Antoine Versini,Arnaud Mansart,Fabien Sindikubwabo,Leeroy Baron,Laila Emam,Pierre Gestraud,G Dan Pantoș,Vincent Gandon,Christine Gaillet,Ting-Di Wu,Florent Dingli,Damarys Loew,Sylvain Baulande,Sylvère Durand,Valentin Sencio,Cyril Robil,François Trottein,David Péricat,Emmanuelle Näser,Céline Cougoule,Etienne Meunier,Anne-Laure Bègue,Hélène Salmon,Nicolas Manel,Alain Puisieux,Sarah Watson,Mark A Dawson,Nicolas Servant,Guido Kroemer,Djillali Annane,Raphaël Rodriguez

Physiology international 109:334-347 PubMed36067021

2022

NCAPH promotes proliferation as well as motility of breast cancer cells by activating the PI3K/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Zhang,Peng Li,Wanying Guo,Qipeng Liu,Weiqiang Qiao,Miao Deng

eLife 9: PubMed32915140

2020

Large domains of heterochromatin direct the formation of short mitotic chromosome loops.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian H Fitz-James,Pin Tong,Alison L Pidoux,Hakan Ozadam,Liyan Yang,Sharon A White,Job Dekker,Robin C Allshire

Cells 9: PubMed31877751

2019

Identification and Characterization of a New Platinum-Induced TP53 Mutation in MDAH Ovarian Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria Lorenzon,Ilenia Pellarin,Ilenia Pellizzari,Sara D'Andrea,Barbara Belletti,Maura Sonego,Gustavo Baldassarre,Monica Schiappacassi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRRN1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-BRRN1 antibody (AB154105)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRRN1 antibody (AB154105)

Western blot - Anti-BRRN1 antibody (AB154105)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise