AB206298

Anti-BRSK1 antibody [EPR18190]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal BRSK1 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

KIAA1811, SAD1, SADB, BRSK1, Serine/threonine-protein kinase BRSK1, Brain-selective kinase 1, Brain-specific serine/threonine-protein kinase 1, Serine/threonine-protein kinase SAD-B, Synapses of Amphids Defective homolog 1, BR serine/threonine-protein kinase 1, SAD1 homolog, hSAD1

5 Images

Immunocytochemistry/ Immunofluorescence - Anti-BRSK1 antibody [EPR18190] (AB206298)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BRSK1 antibody [EPR18190] (AB206298)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cells) cells labeling BRSK1 with ab206298 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows;
-ve control 1 : ab206298 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BRSK1 antibody [EPR18190] (AB206298)

Immunofluorescent analysis of 4% paraformaldehyde-fixed 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embyro fibroblast cells) cells labeling BRSK1 with ab206298 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing both nuclear and cytoplasmic staining on NIH/3T3 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows;
-ve control 1 : ab206298 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution

Supplier Data

Western blot - Anti-BRSK1 antibody [EPR18190] (AB206298)

Blocking/Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-BRSK1 antibody [EPR18190] (ab206298) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 87 kDa

Observed band size: 85 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-BRSK1 antibody [EPR18190] (AB206298)

Exposure time : Lane 1 - 30 seconds; Lane 2 - 3 minutes.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-BRSK1 antibody [EPR18190] (ab206298) at 1/1000 dilution

Lane 1:

PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

Lane 2:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 87 kDa

Observed band size: 85 kDa

false

Supplier Data

Western blot - Anti-BRSK1 antibody [EPR18190] (AB206298)

Blocking/Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-BRSK1 antibody [EPR18190] (ab206298) at 1/1000 dilution

Lane 1:

Rat brain tissue lysate at 10 µg

Lane 2:

Rat heart tissue lysate at 10 µg

Lane 3:

Rat kidney tissue lysate at 10 µg

Lane 4:

Rat spleen tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

Predicted band size: 87 kDa

Observed band size: 85 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18190

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in postmitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma-tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome. Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C.

See full target information BRSK1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 42:112633 PubMed37314927

2023

Phosphatidylinositol 4-kinase IIα is a glycogen synthase kinase 3-regulated interaction hub for activity-dependent bulk endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eva-Maria Blumrich,Jessica C Nicholson-Fish,Marie Pronot,Elizabeth C Davenport,Dominic Kurian,Adam Cole,Karen J Smillie,Michael A Cousin

Journal of neuroinflammation 17:305 PubMed33059746

2020

Forsythoside B attenuates memory impairment and neuroinflammation via inhibition on NF-κB signaling in Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fan'ge Kong,Xue Jiang,Ruochen Wang,Siyu Zhai,Yizhi Zhang,Di Wang

Brazilian journal of medical and biological resear 53:e9175 PubMed32267308

2020

SAD-B modulates epileptic seizure by regulating AMPA receptors in patients with temporal lobe epilepsy and in the PTZ-induced epileptic model.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Miaoqing He,Bing Wu,Peng Zhang,Qinbin Zhang,Yangmei Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BRSK1 antibody [EPR18190]

Immunocytochemistry/ Immunofluorescence - Anti-BRSK1 antibody [EPR18190] (AB206298)

Immunocytochemistry/ Immunofluorescence - Anti-BRSK1 antibody [EPR18190] (AB206298)

Western blot - Anti-BRSK1 antibody [EPR18190] (AB206298)

Western blot - Anti-BRSK1 antibody [EPR18190] (AB206298)

Western blot - Anti-BRSK1 antibody [EPR18190] (AB206298)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise