Rabbit Polyclonal BS69 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZMYND11.
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Publications
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- Cell stem cell 28:877-893.e92021Regional identity of human neural stem cells determines oncogenic responses to histone H3.3 mutants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaul Bardini Bressan,Benjamin Southgate,Kirsty M Ferguson,Carla Blin,Vivien Grant,Neza Alfazema,Jimi C Wills,Maria Angeles Marques-Torrejon,Gillian M Morrison,James Ashmore,Faye Robertson,Charles A C Williams,Leanne Bradley,Alex von Kriegsheim,Richard A Anderson,Simon R Tomlinson,Steven M PollardPubMed 33631116
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ZMYND11. The exact immunogen used to generate this antibody is proprietary information. Database link Q15326
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rhesus monkey, Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rhesus monkey, Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rhesus monkey, Mouse | Dilution info - | Notes - |
Target data
Function
Chromatin reader that specifically recognizes and binds histone H3.3 trimethylated at 'Lys-36' (H3.3K36me3) and regulates RNA polymerase II elongation. Does not bind other histone H3 subtypes (H3.1 or H3.2) (By similarity). Colocalizes with highly expressed genes and functions as a transcription corepressor by modulating RNA polymerase II at the elongation stage. Binds non-specifically to dsDNA (PubMed:24675531). Acts as a tumor-suppressor by repressing a transcriptional program essential for tumor cell growth. (Microbial infection) Inhibits Epstein-Barr virus EBNA2-mediated transcriptional activation and host cell proliferation, through direct interaction.
Alternative names
BRAM1, BS69, ZMYND11, Zinc finger MYND domain-containing protein 11, Adenovirus 5 E1A-binding protein, Bone morphogenetic protein receptor-associated molecule 1, Protein BS69
Recommended products
Rabbit Polyclonal BS69 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ZMYND11.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ZMYND11. The exact immunogen used to generate this antibody is proprietary information. Database link Q15326
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Anti-BS69 antibody (ab227086)
7.5% SDS-PAGE gel.
All lanes: Western blot - Anti-BS69 antibody (ab227086) at 1/1000 dilution
Lane 1: Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2: BS69 transfected HEK-293T cell lysate
Predicted band size: 71 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BS69 antibody (ab227086)
Paraffin-embedded human colon carcinoma tissue stained for BS69 using ab227086 at 1/250 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-BS69 antibody (ab227086)
4% paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for BS69 (green) using ab227086 at 1/500 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
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