Anti-BSCL2/Seipin antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BSCL2/Seipin antibody (AB106793)

IHC image of BSCL2/Seipin staining in human normal testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab106793, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• WB

AbReview44201****

Western blot - Anti-BSCL2/Seipin antibody (AB106793)

All lanes:

Western blot - Anti-BSCL2/Seipin antibody (ab106793) at 1/1000 dilution

Lane 1:

Wild type mouse white adipose tissue lysate (whole) at 50 µg

Lane 2:

Knockout mouse white adipose tissue lysate (whole) at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal

Predicted band size: 44 kDa

Observed band size: 48 kDa

true

Exposure time: 5s

This image is courtesy of an Abreview submitted by Xavier Prieur

• WB

Unknown

Western blot - Anti-BSCL2/Seipin antibody (AB106793)

BSCL2/Seipin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

All lanes:

Western blot - Anti-BSCL2/Seipin antibody (ab106793) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Brain (Rat) Tissue Lysate at 10 µg

Lane 4:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 5:

Testis (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 44 kDa

Observed band size: 100 kDa,46 kDa,75 kDa

true

Exposure time: 3min

• WB

CiteAb

Western blot - Anti-BSCL2/Seipin antibody (AB106793)

BSCL2/Seipin western blot using anti-BSCL2/Seipin antibody ab106793. Publication image and figure legend from Mcilroy, G. D., Suchacki, K., et al., 2018, Mol Metab, PubMed 29459250.

ab106793 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab106793 please see the product overview.

Generation of Ad-B2(-/-)mice. (A) Targeting strategy for the conditional disruption of the Bscl2 gene. Body weights (B), dissection images with magnetic resonance imaging (C), and glucose tolerance (D) of SKO mice (n = 5–6). (E) Body weights and gross morphology of mice after Adiponectin-Cre recombination (n = 7–8). (F) Bscl2 mRNA levels of 16-week-old Ad-B2(+/-) and Ad-B2(-/-) mice in BAT, EWAT, liver, kidney, and heart (n = 4–5). (G) Western blot analysis of Bscl2 protein from BAT of mice described in F (n = 4–5). All data are presented as the mean ± SEM, *p < 0.05.

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