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AB38307

Anti-BSD antibody

3

(2 Reviews)

|

(2 Publications)

Rabbit Polyclonal BSD antibody. Suitable for WB, ICC/IF, ELISA and reacts with Transfected cell line, Aspergillus terreus samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Aspergillus terreus bsd conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Blasticidin-S deaminase, bsd

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-BSD antibody (AB38307)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BSD antibody (AB38307)

ab38307, staining BSD in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were fixed in paraformaldehyde (3.75%) for 30 min., then permeabilized in 0.2% Triton X-100 for 15 min. Blocking was done using 1% BSA for 30 min. The primary was used at 1/100 and then an appropriate secondary antibody conjugated to Texas Red was used.

Image courtesy of Charles Mashburn and Dr. George Smith at the University of Kentucky, Spinal Cord and Brain Injury Research Center

Western blot - Anti-BSD antibody (AB38307)
  • WB

Unknown

Western blot - Anti-BSD antibody (AB38307)

Each lane was loaded with 10-20µg total protein.

All lanes:

Western blot - Anti-BSD antibody (ab38307)

Lane 1:

293 cells mock transfected

Lanes 2 - 3:

293 cells transiently transfected with pLenti6/TR lentiviral vector

Observed band size: 13 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Aspergillus terreus

Applications

ICC/IF, WB, ELISA

applications

Immunogen

Synthetic Peptide within Aspergillus terreus bsd conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P0C2P0

Specificity

The antibody detects a 13 kDa protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Aspergillus terreus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/2000", "ELISA-species-notes": "<p></p>" }, "Transfected cell line": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was affinity purified using the N-terminal Bsd peptide (without carrier).
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the deamination of the cytosine moiety of the antibiotics blasticidin S, cytomycin and acetylblasticidin S.
See full target information bsd

Additional targets

BSD
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 87:5065-80 PubMed23427158

2013

Very-low-density lipoprotein (VLDL)-producing and hepatitis C virus-replicating HepG2 cells secrete no more lipoviroparticles than VLDL-deficient Huh7.5 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Baptiste Jammart,Maud Michelet,Eve-Isabelle Pécheur,Romain Parent,Birke Bartosch,Fabien Zoulim,David Durantel

Journal of virology 86:7072-83 PubMed22532681

2012

Mutagenesis of the DI/DIII linker in dengue virus envelope protein impairs viral particle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Melissanne de Wispelaere,Priscilla L Yang
View all publications
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Product promise

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