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AB242408

Anti-BSND antibody [EPR14270] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal BSND antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

BART, BSND, Barttin

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BSND antibody [EPR14270] - BSA and Azide free (AB242408)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BSND antibody [EPR14270] - BSA and Azide free (AB242408)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling BSND with ab196017 at 1/2000. Secondary antibody : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

Inset image : negative control obtained using PBS instead of ab196017 and secondary antibody only.
Note : Membrane and cytoplasm staining on human kidney tissue was observed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196017)

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BSND antibody [EPR14270] - BSA and Azide free (AB242408)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BSND antibody [EPR14270] - BSA and Azide free (AB242408)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling BSND with ab196017 at 1/2000. Secondary antibody : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

Inset image : negative control obtained using PBS instead of ab196017 and secondary antibody only.
Note : Membrane and cytoplasm staining on rat kidney tissue was observed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196017)

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BSND antibody [EPR14270] - BSA and Azide free (AB242408)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BSND antibody [EPR14270] - BSA and Azide free (AB242408)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling BSND with ab196017 at 1/2000. Secondary antibody : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500.

Inset image : negative control obtained using PBS instead of ab196017 and secondary antibody only.
Note : Membrane and cytoplasm staining on mouse kidney tissue was observed.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196017)

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Unconjugated

    Anti-BSND antibody [EPR14270] - C-terminal

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-BSND antibody [EPR14270] - C-terminal

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-BSND antibody [EPR14270] - C-terminal

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-BSND antibody [EPR14270] - C-terminal

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-BSND antibody [EPR14270] - C-terminal

  • 578 PE

    PE Anti-BSND antibody [EPR14270] - C-terminal

  • 660 APC

    APC Anti-BSND antibody [EPR14270] - C-terminal

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-BSND antibody [EPR14270] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14270

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab242408 is the carrier-free version of ab196017.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulatory subunit of anion-selective CLCNKA : BSND and CLCNKB : BSND heteromeric channels involved in basolateral chloride conductance along the nephron to achieve urine concentration and maintain systemic acid-base homeostasis, and in the stria vascularis of the inner ear to establish the endocochlear potential necessary for normal hearing (PubMed : 11734858, PubMed : 12111250, PubMed : 12574213, PubMed : 16849430, PubMed : 18776122, PubMed : 19646679, PubMed : 20538786, PubMed : 26013830). Most likely acts as a chaperone that allosterically regulates proper sorting of CLCNKA : BSND and CLCNKB : BSND channels at the basolateral plasma membrane domain and functional switch to ion conducting state. Mediates constitutive opening of channel common gates (PubMed : 11734858, PubMed : 12111250, PubMed : 12574213, PubMed : 16849430, PubMed : 18776122, PubMed : 19646679, PubMed : 20538786, PubMed : 26013830).
See full target information BSND
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 11:820 PubMed32625084

2020

BCL11A Is Oncogenic and Predicts Poor Outcomes in Natural Killer/T-Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Shi,Chun Li,Wei Feng,Jianjun Yue,Jingfang Song,Aizhi Peng,Hua Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com