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Rabbit Recombinant Monoclonal BST2/Tetherin antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

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Images

Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (AB243229), expandable thumbnail
  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] (AB243229), expandable thumbnail
  • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (AB243229), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (AB243229), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (AB243229), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted (PubMed:18200009, PubMed:18342597, PubMed:19036818, PubMed:19879838, PubMed:20019814, PubMed:20399176, PubMed:20419159, PubMed:20940320, PubMed:21529378, PubMed:22520941). Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae: hepatitis C virus (HCV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), arenaviridae: lassa virus (LASV) and machupo virus (MACV), herpesviridae: kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae: vesicular stomatitis virus (VSV), orthomyxoviridae: influenza A virus, paramyxoviridae: nipah virus, and coronaviridae: SARS-CoV (PubMed:18200009, PubMed:18342597, PubMed:19179289, PubMed:19879838, PubMed:20399176, PubMed:20419159, PubMed:20686043, PubMed:20943977, PubMed:21529378, PubMed:21621240, PubMed:22520941, PubMed:26378163, PubMed:31199522). Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration (PubMed:22065321). Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection (PubMed:19564354, PubMed:26172439). Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Isoform 1 and isoform 2 are both effective viral restriction factors but have differing antiviral and signaling activities (PubMed:23028328, PubMed:26172439). Isoform 2 is resistant to HIV-1 Vpu-mediated degradation and restricts HIV-1 viral budding in the presence of Vpu (PubMed:23028328, PubMed:26172439). Isoform 1 acts as an activator of NF-kappa-B and this activity is inhibited by isoform 2 (PubMed:23028328).

Alternative names

CD317, Bone marrow stromal antigen 2, BST-2, HM1.24 antigen, Tetherin, BST2

Recommended products

Rabbit Recombinant Monoclonal BST2/Tetherin antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR20202-169
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229), expandable thumbnail

    Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)

    Flow cytometric analysis of U-937 (human histiocytic lymphoma cell line) cell line labeling BST2/Tetherin with ab243229 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229), expandable thumbnail

    Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)

    BST2/Tetherin was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243229 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab243229 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10 μg (input).
    Lane 2: ab243229 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab243229 in HeLa whole cell lysate.

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST
    Exposure time: 3 seconds.

    All lanes: Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)

    Predicted band size: 20 kDa

  • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229), expandable thumbnail

    Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)

    Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling BST2/Tetherin with ab243229 at 1/500 dilution (Red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma cell line) cells labeling BST2/Tetherin with ab243229 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-937 cells (PMID: 20529266).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling BST2/Tetherin with ab243229 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cells (PMID: 20529266).

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229), expandable thumbnail

    Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19737401)

    All lanes: Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229) at 1/1000 dilution

    All lanes: U937 (human histiocytic lymphoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa

    Observed band size: 35 kDa, 70 kDa

  • Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229), expandable thumbnail

    Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229)

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 19196977; PMID: 19737401).

    BST2/Tetherin is a glycosylated protein, its calculated MW is 20kDa, and the observed MW is 35 kDa, which is consistent to the literature.

    Both 35 and 70-kDa bands were detected under the reducing condition, whereas under the non-reducing condition, only the 70-kDa band was detected.

    All lanes: Western blot - Anti-BST2/Tetherin antibody [EPR20202-169] (ab243229) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervix adenocarcinom), whole cell lysate, in the loading buffer containing DTT at 20 µg

    Lane 2: HeLa whole cell lysate in the loading buffer without DTT at 20 µg

    Lane 3: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate, in the loading buffer containing DTT at 20 µg

    Lane 4: K562 whole cell lysate in the loading buffer without DTT at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 20 kDa

    Observed band size: 35 kDa, 70 kDa

    Exposure time: 48s

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Product protocols

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