JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB243563

Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (ab243563) is a rabbit recombinant monoclonal antibody in a PBS only buffer for easy conjugation detecting BST2/Tetherin in Western Blot, Flow Cytometry, IP, ICC/IF. Suitable for Human.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD317, Bone marrow stromal antigen 2, BST-2, HM1.24 antigen, Tetherin, BST2

5 Images
Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)

Flow cytometric analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cell line labeling BST2/Tetherin with ab243229 at 1/500 dilution (Red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243229).

Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)

Flow cytometric analysis of U-937 (human histiocytic lymphoma cell line) cell line labeling BST2/Tetherin with ab243229 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243229).

Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling BST2/Tetherin with ab243229 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HeLa cells (PMID : 20529266).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243229).

Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-937 (human histiocytic lymphoma cell line) cells labeling BST2/Tetherin with ab243229 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-937 cells (PMID : 20529266).

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243229).

Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)
  • IP

Unknown

Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] - BSA and Azide free (AB243563)

BST2/Tetherin was immunoprecipitated from 0.35 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab243229 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab243229 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

Lane 1 : HeLa whole cell lysate 10 μg (input).
Lane 2 : ab243229 IP in HeLa whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab243229 in HeLa whole cell lysate.

Blocking/Diluting buffer and concentration : 5% NFDM/TBST
Exposure time : 3 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243229).

All lanes:

Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR20202-169] (<a href='/en-us/products/primary-antibodies/bst2-tetherin-antibody-epr20202-169-ab243229'>ab243229</a>)

Predicted band size: 20 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20202-169

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
style
left-column

You may be interested in:

AB231931

Human BST2 ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted (PubMed : 18200009, PubMed : 18342597, PubMed : 19036818, PubMed : 19879838, PubMed : 20019814, PubMed : 20399176, PubMed : 20419159, PubMed : 20940320, PubMed : 21529378, PubMed : 22520941). Its target viruses belong to diverse families, including retroviridae : human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae : hepatitis C virus (HCV), filoviridae : ebola virus (EBOV) and marburg virus (MARV), arenaviridae : lassa virus (LASV) and machupo virus (MACV), herpesviridae : kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae : vesicular stomatitis virus (VSV), orthomyxoviridae : influenza A virus, paramyxoviridae : nipah virus, and coronaviridae : SARS-CoV (PubMed : 18200009, PubMed : 18342597, PubMed : 19179289, PubMed : 19879838, PubMed : 20399176, PubMed : 20419159, PubMed : 20686043, PubMed : 20943977, PubMed : 21529378, PubMed : 21621240, PubMed : 22520941, PubMed : 26378163, PubMed : 31199522). Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration (PubMed : 22065321). Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection (PubMed : 19564354, PubMed : 26172439). Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Isoform 1 and isoform 2 are both effective viral restriction factors but have differing antiviral and signaling activities (PubMed : 23028328, PubMed : 26172439). Isoform 2 is resistant to HIV-1 Vpu-mediated degradation and restricts HIV-1 viral budding in the presence of Vpu (PubMed : 23028328, PubMed : 26172439). Isoform 1 acts as an activator of NF-kappa-B and this activity is inhibited by isoform 2 (PubMed : 23028328).
See full target information BST2
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB246508

Anti-BST2/Tetherin antibody [EPR23597-202]

primary-antibodies

bst2-tetherin-antibody-epr23597-202-ab246508

0

(0 reviews)

Alternative Product
Primary Antibodies

AB134061

Anti-BST2/Tetherin antibody [EPR5648]

primary-antibodies

bst2-tetherin-antibody-epr5648-ab134061

4

(5 reviews)

Alternative Product
Primary Antibodies

AB243561

Anti-BST2/Tetherin antibody [EPR20202-150] - BSA and Azide free

primary-antibodies

bst2-tetherin-antibody-epr20202-150-bsa-and-azide-free-ab243561

0

(0 reviews)

Alternative Product
Primary Antibodies

AB272175

Anti-BST2/Tetherin antibody [EPR23597-202] - BSA and Azide free

primary-antibodies

bst2-tetherin-antibody-epr23597-202-bsa-and-azide-free-ab272175

0

(0 reviews)

Alternative Product
Primary Antibodies

AB272176

Anti-BST2/Tetherin antibody [EPR23597-266] - BSA and Azide free

primary-antibodies

bst2-tetherin-antibody-epr23597-266-bsa-and-azide-free-ab272176

0

(0 reviews)

Alternative Product
Primary Antibodies

AB243230

Anti-BST2/Tetherin antibody [EPR20202-150]

primary-antibodies

bst2-tetherin-antibody-epr20202-150-ab243230

0

(0 reviews)

Alternative Product
Primary Antibodies

AB272169

Anti-BST2/Tetherin antibody [EPR23597-266]

primary-antibodies

bst2-tetherin-antibody-epr23597-266-ab272169

0

(0 reviews)

Alternative Product
Primary Antibodies

AB232454

Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free

primary-antibodies

bst2-tetherin-antibody-epr5648-bsa-and-azide-free-ab232454

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com