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Rabbit Recombinant Monoclonal BST2/Tetherin antibody. Suitable for IHC-P, IP, Flow Cyt, ICC/IF and reacts with Mouse samples. Cited in 1 publication.

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Images

Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-202] (AB246508), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] (AB246508), expandable thumbnail
  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] (AB246508), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] (AB246508), expandable thumbnail
  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] (AB246508), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPFlow CytWBICC/IF
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Tested
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), mouse mammary tumor virus (MMTV) and murine leukemia virus (MLV), filoviridae: ebola virus (EBOV), arenaviridae: lassa virus (LASV), and rhabdoviridae: vesicular stomatitis virus (VSV). Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration. Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection. Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells.

Alternative names

CD317, Bone marrow stromal antigen 2, BST-2, HM1.24 antigen, Bst2

Recommended products

Rabbit Recombinant Monoclonal BST2/Tetherin antibody. Suitable for IHC-P, IP, Flow Cyt, ICC/IF and reacts with Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23597-202
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508), expandable thumbnail

    Flow Cytometry - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508)

    Flow cytometric analysis of Mouse splenocyte cells labelling BST2/Tetherin with ab246508 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left).A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were stained with rabbit IgG (Left) or ab246508 (Right). Then stained with anti-CD45R conjugated to Alexa Fluor® 647.

    Gated on viable cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508)

    Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling BST2/Tetherin with ab246508 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse spleen (PMID: 19903902).The section was incubated with ab246508 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508), expandable thumbnail

    Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508)

    BST2/Tetherin was immunoprecipitated from 0.35 mg EL4 (mouse lymphoma T lymphocyte) whole cell lysate with ab246508 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246508 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: EL4 (mouse lymphoma T lymphocyte), whole cell lysate 10 ug

    Lane 2: ab246508 IP in EL4 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab246508 in EL4 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, which is consistent to the literature(PMID: 22520941; PMID: 19737401).

    All lanes: Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508)

    Predicted band size: 20 kDa

    Observed band size: 28-40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Mouse splenocyte cells labelling BST2/Tetherin with ab246508 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in mouse splenocyte Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508), expandable thumbnail

    Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508)

    BST2/Tetherin was immunoprecipitated from 0.35 mg Mouse spleen tissue lysate 10ug with ab246508 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246508 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution. Lane 1: Mouse spleen tissue lysate 10ug

    Lane 2: ab246508 IP in Mouse spleentissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab246508 in mouse spleen tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 15 seconds

    BST2 is type II transmembrane glycoprotein with a molecular mass of 28-40 KD, which is consistent to the literature(PMID: 22520941; PMID: 19737401).

    All lanes: Immunoprecipitation - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508)

    Predicted band size: 20 kDa

    Observed band size: 28-40 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508)

    Immunofluorescent analysis of 100% methanol-fixed EL.4 cells labelling BST2/Tetherin with ab246508 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in EL.4 cell line. 100% methanol fixation is recommended. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BST2/Tetherin antibody [EPR23597-202] (ab246508)

    Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue labeling BST2/Tetherin with ab246508 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse skeletal muscle (PMID: 19903902). The section was incubated with ab246508 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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