AB134061

Anti-BST2/Tetherin antibody [EPR5648]

Name: Anti-BST2/Tetherin antibody [EPR5648] (ab134061) | Abcam
Brand: Abcam Limited
SKU: AB134061
Price: 650 USD
Availability: InStock
Rating: 4 (5 reviews)

RabMAb
Recombinant
KO Validated
What is this?

(5 Reviews)

(6 Publications)

Rabbit Recombinant Monoclonal BST2/Tetherin antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications.

View Alternative Names

CD317, Bone marrow stromal antigen 2, BST-2, HM1.24 antigen, Tetherin, BST2

4 Images

Unknown

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

All lanes:

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 28-40 kDa

false

Lab

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

Blocking/Diluting buffer and concentration 5% NFDM/TBST

This antibody detects a non-specific band at 65 KDa.

This image was produced using unpurified antibody.

All lanes:

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

All lanes:

Human fetal spleen whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 20 kDa

Observed band size: 30 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

ab134061 was shown to react with BST2 in wild-type A-431 cells in western blot. Loss of signal was observed when BST2 knockout cell line ab261907 (knockout cell lysate ab261713) was used. Membranes were blocked in 3% milk in TBS-T (0.1% Tween^®) before incubation with ab134061 overnight at 4°C at a 1 in 1000 dilution and ab184095 (Mouse Anti-GAPDH antibody [mAbcam 9484] - Alexa Fluor^® 680) at a 1 in 1000 dilution. Blots were incubated with HRP conjugated Goat anti-Rabbit (H+L) secondary antibody at 1/5000 for 1 hour at room temperature before development with Optiblot ECL reagent (ab133456) and imaging.

All lanes:

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

Lane 1:

Wild-type A431 cell lysate at 40 µg

Lane 2:

BST2 knockout A431 cell lysate at 40 µg

Lane 2:

Western blot - Human BST2 (Tetherin) knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-bst2-tetherin-knockout-a-431-cell-line-ab261907'>ab261907</a>)

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 40 µg

Lane 4:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate at 40 µg

Predicted band size: 20 kDa

Observed band size: 30 kDa

false

Exposure time: 20min

Unknown

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

This image was produced using unpurified antibody.

All lanes:

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (ab134061) at 1/1000 dilution

Lane 1:

U937 lysate at 10 µg

Lane 2:

Molt-4 lysate at 10 µg

Lane 3:

Human spleen lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 20 kDa

false

Carrier free

Anti-BST2/Tetherin antibody [EPR5648] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5648

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

For Mouse and Rat species, we have preliminary internal testing data to indicate this antibody may not react. Please contact us for more information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Stable for 12 months at -20°C

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted (PubMed : 18200009, PubMed : 18342597, PubMed : 19036818, PubMed : 19879838, PubMed : 20019814, PubMed : 20399176, PubMed : 20419159, PubMed : 20940320, PubMed : 21529378, PubMed : 22520941). Its target viruses belong to diverse families, including retroviridae : human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae : hepatitis C virus (HCV), filoviridae : ebola virus (EBOV) and marburg virus (MARV), arenaviridae : lassa virus (LASV) and machupo virus (MACV), herpesviridae : kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae : vesicular stomatitis virus (VSV), orthomyxoviridae : influenza A virus, paramyxoviridae : nipah virus, and coronaviridae : SARS-CoV (PubMed : 18200009, PubMed : 18342597, PubMed : 19179289, PubMed : 19879838, PubMed : 20399176, PubMed : 20419159, PubMed : 20686043, PubMed : 20943977, PubMed : 21529378, PubMed : 21621240, PubMed : 22520941, PubMed : 26378163, PubMed : 31199522). Can inhibit cell surface proteolytic activity of MMP14 causing decreased activation of MMP15 which results in inhibition of cell growth and migration (PubMed : 22065321). Can stimulate signaling by LILRA4/ILT7 and consequently provide negative feedback to the production of IFN by plasmacytoid dendritic cells in response to viral infection (PubMed : 19564354, PubMed : 26172439). Plays a role in the organization of the subapical actin cytoskeleton in polarized epithelial cells. Isoform 1 and isoform 2 are both effective viral restriction factors but have differing antiviral and signaling activities (PubMed : 23028328, PubMed : 26172439). Isoform 2 is resistant to HIV-1 Vpu-mediated degradation and restricts HIV-1 viral budding in the presence of Vpu (PubMed : 23028328, PubMed : 26172439). Isoform 1 acts as an activator of NF-kappa-B and this activity is inhibited by isoform 2 (PubMed : 23028328).

See full target information BST2

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cells 10: PubMed33800686

2021

Parkinson's Disease Causative Mutation in Vps35 Disturbs Tetherin Trafficking to Cell Surfaces and Facilitates Virus Spread.

Applications

Unspecified application

Species

Unspecified reactive species

Yingzhuo Ding,Yan Li,Gaurav Chhetri,Xiaoxin Peng,Jing Wu,Zejian Wang,Bo Zhao,Wenjuan Zhao,Xueyi Li

Cell reports 26:3257-3271.e8 PubMed30893599

2019

Nidogen-1 Contributes to the Interaction Network Involved in Pro-B Cell Retention in the Peri-sinusoidal Hematopoietic Stem Cell Niche.

Applications

Unspecified application

Species

Unspecified reactive species

Marielle Balzano,Maria De Grandis,Thien-Phong Vu Manh,Lionel Chasson,Florence Bardin,Anne Farina,Arnauld Sergé,Ghislain Bidaut,Pierre Charbord,Léonard Hérault,Anne-Laure Bailly,Amandine Cartier-Michaud,Annie Boned,Marc Dalod,Estelle Duprez,Paul Genever,Mark Coles,Marc Bajenoff,Luc Xerri,Michel Aurrand-Lions,Claudine Schiff,Stéphane J C Mancini

Cancer research 79:2220-2231 PubMed30890618

2019

CD317 Activates EGFR by Regulating Its Association with Lipid Rafts.

Applications

Unspecified application

Species

Unspecified reactive species

Guizhong Zhang,Xin Li,Qian Chen,Junxin Li,Qingguo Ruan,Youhai H Chen,Xiaolu Yang,Xiaochun Wan

Journal of molecular biology 430:3111-3128 PubMed30131116

2018

DDX17 Specifically, and Independently of DDX5, Controls Use of the HIV A4/5 Splice Acceptor Cluster and Is Essential for Efficient Replication of HIV.

Applications

Unspecified application

Species

Unspecified reactive species

Nyaradzai Sithole,Claire A Williams,Aisling M Vaughan,Julia C Kenyon,Andrew M L Lever

Virology 513:11-16 PubMed29028477

2017

Modulation of the NF-κB signaling pathway by the HIV-2 envelope glycoprotein and its incomplete BST-2 antagonism.

Applications

Species

Unspecified reactive species

François E Dufrasne,Mara Lucchetti,Anandi Martin,Emmanuel André,Géraldine Dessilly,Benoit Kabamba,Patrick Goubau,Jean Ruelle

Journal of experimental & clinical cancer research 35:117 PubMed27444183

2016

CD317 Promotes the survival of cancer cells through apoptosis-inducing factor.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Guizhong Zhang,Qian Chen,Yingxue Lin,Junxin Li,Qingguo Ruan,Youhai Chen,Guang Yu,Xiaochun Wan

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BST2/Tetherin antibody [EPR5648]

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

Western blot - Anti-BST2/Tetherin antibody [EPR5648] (AB134061)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise