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AB72285

Anti-BTAF1 antibody

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(1 Publication)

Rabbit Polyclonal BTAF1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BTAF1 aa 50-150.

View Alternative Names

TAF172, BTAF1, TATA-binding protein-associated factor 172, ATP-dependent helicase BTAF1, B-TFIID transcription factor-associated 170 kDa subunit, TAF(II)170, TBP-associated factor 172, TAF-172

2 Images
Immunoprecipitation - Anti-BTAF1 antibody (AB72285)
  • IP

Unknown

Immunoprecipitation - Anti-BTAF1 antibody (AB72285)

Detection of Human BTAF1 by Immunoprecipitation in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab72285 at 3μg/mg lysate for IP and at 1μg/ml for subsequent WB.

All lanes:

Immunoprecipitation - Anti-BTAF1 antibody (ab72285)

Predicted band size: 207 kDa

false

Western blot - Anti-BTAF1 antibody (AB72285)
  • WB

Unknown

Western blot - Anti-BTAF1 antibody (AB72285)

All lanes:

Western blot - Anti-BTAF1 antibody (ab72285) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 207 kDa

Observed band size: 170 kDa,207 kDa,85 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human BTAF1 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

O14981

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.
See full target information BTAF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 67:594-607.e4 PubMed28735899

2017

Mot1, Ino80C, and NC2 Function Coordinately to Regulate Pervasive Transcription in Yeast and Mammals.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Xue,Suman K Pradhan,Fei Sun,Constantinos Chronis,Nancy Tran,Trent Su,Christopher Van,Ajay Vashisht,James Wohlschlegel,Craig L Peterson,H T Marc Timmers,Siavash K Kurdistani,Michael F Carey
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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