AB50990

Anti-BTAF1 antibody [BTA3D61]

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Mouse Monoclonal BTAF1 antibody BTA3D61. Suitable for ICC, IP, Flow Cyt, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

TAF172, BTAF1, TATA-binding protein-associated factor 172, ATP-dependent helicase BTAF1, B-TFIID transcription factor-associated 170 kDa subunit, TAF(II)170, TBP-associated factor 172, TAF-172

6 Images

Flow Cytometry - Anti-BTAF1 antibody [BTA3D61] (AB50990)

Flow Cyt

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Flow Cytometry - Anti-BTAF1 antibody [BTA3D61] (AB50990)

Cells labelled with ab50990 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/400 dilution.

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Immunocytochemistry - Anti-BTAF1 antibody [BTA3D61] (AB50990)

HeLa cells stained with ab50990 at 1/10 dilution followed by Alexa Fluor 488 Goat Anti-Mouse IgG at 1/200.

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Immunoprecipitation - Anti-BTAF1 antibody [BTA3D61] (AB50990)

ab50990 used at 5µg/0.5mg RIPA lysate of HeLa cells. The immunoprecipitate was subjected to Western blotting using ab50990 at 1/100. The ECL technique was used, and the secondary antibody was Mouse IgG at 1/2500.

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Immunoprecipitation - Anti-BTAF1 antibody [BTA3D61] (ab50990)

Predicted band size: 207 kDa

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Western blot - Anti-BTAF1 antibody [BTA3D61] (AB50990)

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Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution

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HEK293 whole cell lysate at 50 µg

Secondary

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Mouse IgG antibody at 1/2500 dilution

Predicted band size: 207 kDa

Observed band size: 207 kDa

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Western blot - Anti-BTAF1 antibody [BTA3D61] (AB50990)

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Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution

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F2408 whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 207 kDa

Observed band size: 207 kDa

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Western blot - Anti-BTAF1 antibody [BTA3D61] (AB50990)

All lanes:

Western blot - Anti-BTAF1 antibody [BTA3D61] (ab50990) at 1/50 dilution

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NIH3T3 whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 207 kDa

Observed band size: 207 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

BTA3D61

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC, WB, IP, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human BTAF1.

O14981

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1/10", "ICC-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use at 5μg/0.5mg of lysate.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "" }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "" }, "Rat": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner.

See full target information BTAF1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BTAF1 antibody [BTA3D61]

Flow Cytometry - Anti-BTAF1 antibody [BTA3D61] (AB50990)

Immunocytochemistry - Anti-BTAF1 antibody [BTA3D61] (AB50990)

Immunoprecipitation - Anti-BTAF1 antibody [BTA3D61] (AB50990)

Western blot - Anti-BTAF1 antibody [BTA3D61] (AB50990)

Western blot - Anti-BTAF1 antibody [BTA3D61] (AB50990)

Western blot - Anti-BTAF1 antibody [BTA3D61] (AB50990)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Product promise