Anti-BTF antibody [EPR9980(2)] (ab181240)

Rabbit Recombinant Monoclonal BTF antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-BTF antibody [EPR9980(2)] (AB181240), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	ICC/IF	IHC-P
Human	Tested	Tested	Tested
Mouse	Tested	Expected	Tested
Rat	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/10000 - 1/50000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/10000 - 1/50000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/10000 - 1/50000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes For unpurified use at 1/500

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes For unpurified use at 1/100 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes For unpurified use at 1/100 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes For unpurified use at 1/100 Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information BCLAF1

Function

Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.

Alternative names

BTF, KIAA0164, BCLAF1, Bcl-2-associated transcription factor 1, Btf, BCLAF1 and THRAP3 family member 1

Recommended products

Rabbit Recombinant Monoclonal BTF antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR9980(2)
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The biological target BTF also known as BTF band or BTF 2 is a protein with an approximate mass of 42 kDa. It is highly expressed in various tissues including the brain and liver indicating its wide importance in cellular functions. BTF operates mechanically as a transcriptional regulator binding DNA sequences to control the transcription of specific genes. Additionally it interacts with other transcription factors assisting in the precise modulation of gene expression.

Biological function summary

BTF plays important roles in cellular proliferation and differentiation often as part of a larger transcriptional regulatory complex. Its activity helps to control gene expression during cell cycle progression and differentiation processes. The protein is essential for the accurate functioning of transcriptional machinery often being involved in the assembly of transcription pre-initiation complexes and modulating RNA polymerase activity.

Pathways

BTF acts centrally in the regulation of two significant pathways: the Wnt signaling pathway and the NF-kB signaling pathway. In both pathways BTF modulates the downstream transcription of responsive genes via its interaction with other pathway proteins like Beta-catenin in Wnt and RelA in NF-kB. These interactions help maintain proper cellular responses to extracellular signals ensuring balanced pathway activity and cellular responses.

Associated diseases and disorders

BTF involvement has been linked to cancer and neurodegenerative diseases. Its dysregulation in transcriptional control can lead to the aberrant expression of genes related to oncogenesis or neuronal function. In cancer alterations in BTF activity may contribute to tumor progression and resistance to apoptosis through its connection with proteins like p53. In neurodegenerative diseases misregulation may affect neuronal growth and survival often interacting with proteins like tau which are linked to these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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9 product images

Western blot - Anti-BTF antibody [EPR9980(2)] (ab181240)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-BTF antibody [EPR9980(2)] (ab181240) at 1.5 µg/mL
Lane 1: K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg
Lane 2: RAW64.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 15 µg
Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 150 kDa
Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] (ab181240)
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling BTF with Purified ab181240 at 1:1000 dilution (1.5 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling BTF with Purified ab181240 at 1:500 dilution (3.08 µg/ml). Heat mediated antigen retrieval was performed using using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] (ab181240)
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling BTF with unpurified ab181240 at 1/500. Cells were fixed with 4% Paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Control: PBS only.
Nuclear counter stain: DAPI.
Western blot - Anti-BTF antibody [EPR9980(2)] (ab181240)
All lanes: Western blot - Anti-BTF antibody [EPR9980(2)] (ab181240) at 1/10000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: K562 cell lysate at 20 µg
Lane 3: MCF-7 cell lysate at 20 µg
Secondary
Lane 1: Goat anti-Rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Lanes 2 - 3: Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 106 kDa
Observed band size: 150 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BTF with unpurified ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] (ab181240)
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling BTF with unpurified ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.