Anti-BTF antibody [EPR9980(2)] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling BTF with Purified ab181240 at 1 : 1000 dilution (1.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling BTF with unpurified ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling BTF with Purified ab181240 at 1 : 500 dilution (3.08 μg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

Negative control : PBS instead of the primary antibody.

Hematoxylinwas used as a counterstain

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BTF with ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)

Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling BTF with unpurified ab181240 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Control : PBS only.
Nuclear counter stain : DAPI.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling BTF with Purified ab181240 at 1 : 500 dilution (3.08 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

Negative control : PBS instead of the primary antibody.

Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling BTF with Purified ab181240 at 1 : 500 dilution (3.08 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.

Negative control : PBS instead of the primary antibody.

Hematoxylin was used as a counterstain

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181240).