Rabbit Recombinant Monoclonal BTF antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunocytochemistry-immunofluorescence-img110062.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107550.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43550.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128304.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)")
![Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunocytochemistry-immunofluorescence-img30262.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (AB236159)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Expected | Tested | Tested |
| Mouse | Expected | Predicted | Predicted |
| Rat | Expected | Predicted | Predicted |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
Alternative names
BTF, KIAA0164, BCLAF1, Bcl-2-associated transcription factor 1, Btf, BCLAF1 and THRAP3 family member 1
Recommended products
Rabbit Recombinant Monoclonal BTF antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Rat, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR9980(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab236159 is the carrier-free version of Anti-BTF antibody [EPR9980(2)] ab181240.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The biological target BTF also known as BTF band or BTF 2 is a protein with an approximate mass of 42 kDa. It is highly expressed in various tissues including the brain and liver indicating its wide importance in cellular functions. BTF operates mechanically as a transcriptional regulator binding DNA sequences to control the transcription of specific genes. Additionally it interacts with other transcription factors assisting in the precise modulation of gene expression.
- Biological function summary
BTF plays important roles in cellular proliferation and differentiation often as part of a larger transcriptional regulatory complex. Its activity helps to control gene expression during cell cycle progression and differentiation processes. The protein is essential for the accurate functioning of transcriptional machinery often being involved in the assembly of transcription pre-initiation complexes and modulating RNA polymerase activity.
- Pathways
BTF acts centrally in the regulation of two significant pathways: the Wnt signaling pathway and the NF-kB signaling pathway. In both pathways BTF modulates the downstream transcription of responsive genes via its interaction with other pathway proteins like Beta-catenin in Wnt and RelA in NF-kB. These interactions help maintain proper cellular responses to extracellular signals ensuring balanced pathway activity and cellular responses.
- Associated diseases and disorders
BTF involvement has been linked to cancer and neurodegenerative diseases. Its dysregulation in transcriptional control can lead to the aberrant expression of genes related to oncogenesis or neuronal function. In cancer alterations in BTF activity may contribute to tumor progression and resistance to apoptosis through its connection with proteins like p53. In neurodegenerative diseases misregulation may affect neuronal growth and survival often interacting with proteins like tau which are linked to these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunocytochemistry-immunofluorescence-img110062.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)")
Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling BTF with Purified Anti-BTF antibody [EPR9980(2)] ab181240 at 1:1000 dilution (1.5 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BTF antibody [EPR9980(2)] ab181240).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img107550.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling BTF with Purified Anti-BTF antibody [EPR9980(2)] ab181240 at 1:500 dilution (3.08 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody.
Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BTF antibody [EPR9980(2)] ab181240).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img43550.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling BTF with Purified Anti-BTF antibody [EPR9980(2)] ab181240 at 1:500 dilution (3.08 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.
Negative control: PBS instead of the primary antibody.
Hematoxylin was used as a counterstain
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BTF antibody [EPR9980(2)] ab181240).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img128304.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human gastric cancer tissue sections labeling BTF with Purified Anti-BTF antibody [EPR9980(2)] ab181240 at 1:500 dilution (3.08 μg/ml). Heat mediated antigen retrieval was performed using using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.
Negative control: PBS instead of the primary antibody.
Hematoxylinwas used as a counterstain
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BTF antibody [EPR9980(2)] ab181240).
![Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunocytochemistry-immunofluorescence-img30262.jpg "Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)")
Immunocytochemistry/ Immunofluorescence - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling BTF with unpurified Anti-BTF antibody [EPR9980(2)] ab181240 at 1/500. Cells were fixed with 4% Paraformaldehyde. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BTF antibody [EPR9980(2)] ab181240).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108468.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling BTF with Anti-BTF antibody [EPR9980(2)] ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BTF antibody [EPR9980(2)] ab181240).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110092.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)
Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling BTF with unpurified Anti-BTF antibody [EPR9980(2)] ab181240 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-BTF antibody [EPR9980(2)] ab181240).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108468.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159), expandable thumbnail](https://content.abcam.com/products/images/btf-antibody-epr99802-bsa-and-azide-free-ab236159--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img110092.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTF antibody [EPR9980(2)] - BSA and Azide free (ab236159)")
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