AB155965

Anti-BTF antibody [EPR9981]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal BTF antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BTF, KIAA0164, BCLAF1, Bcl-2-associated transcription factor 1, Btf, BCLAF1 and THRAP3 family member 1

2 Images

Flow Cytometry (Intracellular) - Anti-BTF antibody [EPR9981] (AB155965)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BTF antibody [EPR9981] (AB155965)

Intracellular Flow Cytometry analysis of permeabilized K562 cells using ab155965 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

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Western blot - Anti-BTF antibody [EPR9981] (AB155965)

All lanes:

Western blot - Anti-BTF antibody [EPR9981] (ab155965) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 106 kDa

false

Carrier free

Anti-BTF antibody [EPR9981] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9981

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target BTF also known as BTF band or BTF 2 is a protein with an approximate mass of 42 kDa. It is highly expressed in various tissues including the brain and liver indicating its wide importance in cellular functions. BTF operates mechanically as a transcriptional regulator binding DNA sequences to control the transcription of specific genes. Additionally it interacts with other transcription factors assisting in the precise modulation of gene expression.

Biological function summary

BTF plays important roles in cellular proliferation and differentiation often as part of a larger transcriptional regulatory complex. Its activity helps to control gene expression during cell cycle progression and differentiation processes. The protein is essential for the accurate functioning of transcriptional machinery often being involved in the assembly of transcription pre-initiation complexes and modulating RNA polymerase activity.

Pathways

BTF acts centrally in the regulation of two significant pathways: the Wnt signaling pathway and the NF-kB signaling pathway. In both pathways BTF modulates the downstream transcription of responsive genes via its interaction with other pathway proteins like Beta-catenin in Wnt and RelA in NF-kB. These interactions help maintain proper cellular responses to extracellular signals ensuring balanced pathway activity and cellular responses.

BTF involvement has been linked to cancer and neurodegenerative diseases. Its dysregulation in transcriptional control can lead to the aberrant expression of genes related to oncogenesis or neuronal function. In cancer alterations in BTF activity may contribute to tumor progression and resistance to apoptosis through its connection with proteins like p53. In neurodegenerative diseases misregulation may affect neuronal growth and survival often interacting with proteins like tau which are linked to these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.

See full target information BCLAF1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Archives of toxicology 99:1187-1202 PubMed39827450

2025

BMP6 participates in the molecular mechanisms involved in APAP hepatotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Marañón,Stephania C Isaza,Esther Rey,Patricia Rada,Yaiza García-García,James W Dear,Carmelo García-Monzón,Ángela M Valverde,Javier Egea,Águeda González-Rodríguez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BTF antibody [EPR9981]

Flow Cytometry (Intracellular) - Anti-BTF antibody [EPR9981] (AB155965)

Western blot - Anti-BTF antibody [EPR9981] (AB155965)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise