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AB249218

Anti-BTF antibody [EPR9981] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal BTF antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BTF, KIAA0164, BCLAF1, Bcl-2-associated transcription factor 1, Btf, BCLAF1 and THRAP3 family member 1

2 Images
Flow Cytometry (Intracellular) - Anti-BTF antibody [EPR9981] - BSA and Azide free (AB249218)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-BTF antibody [EPR9981] - BSA and Azide free (AB249218)

This data was developed using ab155965, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of permeabilized K562 cells using ab155965 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-BTF antibody [EPR9981] - BSA and Azide free (AB249218)
  • WB

Unknown

Western blot - Anti-BTF antibody [EPR9981] - BSA and Azide free (AB249218)

This data was developed using ab155965, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BTF antibody [EPR9981] (<a href='/en-us/products/primary-antibodies/btf-antibody-epr9981-ab155965'>ab155965</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

MCF-7 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 106 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9981

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249218 is the carrier-free version of ab155965.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target BTF also known as BTF band or BTF 2 is a protein with an approximate mass of 42 kDa. It is highly expressed in various tissues including the brain and liver indicating its wide importance in cellular functions. BTF operates mechanically as a transcriptional regulator binding DNA sequences to control the transcription of specific genes. Additionally it interacts with other transcription factors assisting in the precise modulation of gene expression.
Biological function summary

BTF plays important roles in cellular proliferation and differentiation often as part of a larger transcriptional regulatory complex. Its activity helps to control gene expression during cell cycle progression and differentiation processes. The protein is essential for the accurate functioning of transcriptional machinery often being involved in the assembly of transcription pre-initiation complexes and modulating RNA polymerase activity.

Pathways

BTF acts centrally in the regulation of two significant pathways: the Wnt signaling pathway and the NF-kB signaling pathway. In both pathways BTF modulates the downstream transcription of responsive genes via its interaction with other pathway proteins like Beta-catenin in Wnt and RelA in NF-kB. These interactions help maintain proper cellular responses to extracellular signals ensuring balanced pathway activity and cellular responses.

BTF involvement has been linked to cancer and neurodegenerative diseases. Its dysregulation in transcriptional control can lead to the aberrant expression of genes related to oncogenesis or neuronal function. In cancer alterations in BTF activity may contribute to tumor progression and resistance to apoptosis through its connection with proteins like p53. In neurodegenerative diseases misregulation may affect neuronal growth and survival often interacting with proteins like tau which are linked to these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Death-promoting transcriptional repressor. May be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA.
See full target information BCLAF1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com