JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB244260

Anti-BTG2/PC3 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal BTG2/PC3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BTG2 aa 1-150.

View Alternative Names

PC3, BTG2, Protein BTG2, BTG family member 2, NGF-inducible anti-proliferative protein PC3

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-BTG2/PC3 antibody (AB244260)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BTG2/PC3 antibody (AB244260)

PFA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling BTG2/PC3 using ab244260 at 4 μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTG2/PC3 antibody (AB244260)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTG2/PC3 antibody (AB244260)

Formalin-fixed, paraffin-embedded human cerebellum tissue stained for BTG2/PC3 with ab244260 at a 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BTG2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P78543

style
left-column

Alternative Products

Primary Antibodies

AB85051

Anti-BTG2/PC3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTG2/PC3 antibody (AB85051)

  • 3
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB29

Anti-PCNA antibody [PC10]

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PCNA antibody [PC10] (AB29)

  • 5
  • 72
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BTG2 also known as PC3 is a protein encoded by the BTG2 gene. The protein has a molecular weight of approximately 18-20 kDa. It mainly functions as a tumor suppressor and is involved in the regulation of the cell cycle. This protein modulates the transcriptional activity of genes associated with cell growth and differentiation. BTG2 is broadly expressed in various tissues including the brain liver and spleen where it contributes to maintaining normal cellular functions.
Biological function summary

The BTG2 protein plays an important role in controlling cell cycle progression and apoptosis. It negatively regulates the transition from the G1 phase to the S phase of the cell cycle by interacting with cyclin D1 and E2F-1 transcription factors which are critical for DNA synthesis initiation. Additionally BTG2 does not operate as part of a larger protein complex but can impact the activity and stability of other transcription factors and proteins. Its ability to modulate different cellular processes highlights its importance in maintaining normal cell function and preventing uncontrolled proliferation.

Pathways

BTG2 is integrated into the tumor suppressor network and influences gene transcription and mRNA degradation pathways. It is connected to the p53 pathway where it functions downstream of p53 a protein that responds to stress signals and maintains genomic stability. BTG2's relationship with the p53 pathway also aligns it with proteins such as MDM2 which helps regulate p53 turnover. This pathway connection showcases BTG2's role in mediating DNA damage responses and maintaining cellular integrity.

BTG2's tumor-suppressive functions associate it particularly with cancer. Abnormal expression or mutations in the BTG2 gene can contribute to the progression of breast and prostate cancers. The loss of BTG2 function leads to unregulated cell proliferation aiding cancer development. Furthermore BTG2's connection to the p53 protein and the latter's involvement in multiple types of cancers establishes a critical link in tumor suppression mechanisms highlighting potential therapeutic targets where BTG2 might play a role.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Anti-proliferative protein; the function is mediated by association with deadenylase subunits of the CCR4-NOT complex. Activates mRNA deadenylation in a CNOT6 and CNOT7-dependent manner. In vitro can inhibit deadenylase activity of CNOT7 and CNOT8. Involved in cell cycle regulation. Could be involved in the growth arrest and differentiation of the neuronal precursors (By similarity). Modulates transcription regulation mediated by ESR1. Involved in mitochondrial depolarization and neurite outgrowth.
See full target information BTG2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 31:505-514 PubMed37415745

2023

Betulinic acid-mediating miRNA-365 inhibited the progression of pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Wenkai Jiang,Wancheng Li,Shi Dong,Yan DU,Hui Zhang,Wence Zhou
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com