AB235085

Anti-BTG4 antibody

Name: Anti-BTG4 antibody (ab235085) | Abcam
Brand: Abcam Limited
SKU: AB235085
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal BTG4 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BTG4 aa 1 to C-terminus.

View Alternative Names

PC3B, BTG4, Protein BTG4, BTG family member 4, Protein PC3b

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTG4 antibody (AB235085)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTG4 antibody (AB235085)

Paraffin-embedded human ovarian cancer tissue stained for BTG4 using ab235085 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTG4 antibody (AB235085)

Paraffin-embedded human testis tissue stained for BTG4 using ab235085 at 1/100 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-BTG4 antibody (AB235085)

All lanes:

Western blot - Anti-BTG4 antibody (ab235085) at 1/500 dilution

All lanes:

Mouse brain lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human BTG4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NY30

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purity >95%

Storage buffer

pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BTG4 also known as B-cell translocation gene 4 is a protein with a molecular mass of approximately 26 kDa. This protein is part of the BTG/Tob family which is involved in cell growth regulation. BTG4 is expressed predominantly in testicular tissue where it plays a role in germ cell development. Researchers also find it in lower amounts in other tissues such as the brain and ovary indicating a broader but less pronounced expression profile.

Biological function summary

BTG4 associates with several cellular processes including cell cycle regulation and transcriptional control. It does not operate as an isolated unit but forms complexes with elements like protein arginine methyltransferase suggesting functions in post-translational modifications. BTG4 influences mRNA deadenylation impacting mRNA stability and translation rates processes critical for cellular and developmental adjustments.

Pathways

BTG4 plays a role in the cell cycle and mRNA degradation pathways. Its interaction with proteins like cyclin B1 and Cdc2 highlights its position in the regulation of cellular proliferation and division cycles. As part of these pathways BTG4 modulates key transitions in the cell cycle that ensure proper cellular functions and development.

Disruption in BTG4 expression associates with infertility and certain cancers particularly testicular cancer. This relationship suggests BTG4's involvement in germ cell regulation failures. The association with proteins such as p53 in cancer pathways also illustrates its potential role in tumorigenesis through the influence on cell cycle checkpoints and apoptotic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adapter protein that bridges CNOT7, a catalytic subunit of the CCR4-NOT complex, to EIF4E (By similarity). Facilitates maternal mRNAs decay during the maturation of oocytes and in the fertilized egg, and is required for the maternal-zygotic transition (MZT), zygotic cleavage and initiation of embryonic development (PubMed : 32502391).

See full target information BTG4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:3729 PubMed37349316

2023

Maternal NAT10 orchestrates oocyte meiotic cell-cycle progression and maturation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Jiang,Yu Cheng,Yuzhang Zhu,Caoling Xu,Qiaodan Li,Xuemei Xing,Wenqing Li,Jiaqi Zou,Lan Meng,Muhammad Azhar,Yuzhu Cao,Xianhong Tong,Weibing Qin,Xiaoli Zhu,Jianqiang Bao

Autophagy 19:1781-1802 PubMed36541703

2022

PEX13 prevents pexophagy by regulating ubiquitinated PEX5 and peroxisomal ROS.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas D Demers,Victoria Riccio,Doo Sin Jo,Sushil Bhandari,Kelsey B Law,Weifang Liao,Choy Kim,G Angus McQuibban,Seong-Kyu Choe,Dong-Hyung Cho,Peter K Kim

American journal of human genetics 107:24-33 PubMed32502391

2020

Homozygous Mutations in BTG4 Cause Zygotic Cleavage Failure and Female Infertility.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zheng,Zhou Zhou,Qianqian Sha,Xiangli Niu,Xiaoxi Sun,Juanzi Shi,Lei Zhao,Shuoping Zhang,Jing Dai,Sufen Cai,Fei Meng,Liang Hu,Fei Gong,Xiaoran Li,Jing Fu,Rong Shi,Guangxiu Lu,Biaobang Chen,Hengyu Fan,Lei Wang,Ge Lin,Qing Sang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BTG4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTG4 antibody (AB235085)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTG4 antibody (AB235085)

Western blot - Anti-BTG4 antibody (AB235085)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise