Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (AB251461)

This data was developed using ab206914, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling BTG4 with ab206914 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).

Confocal image showing nuclear and weakly cytoplasmic staining on HeLa cell transfected with GFP-BTG4.

The nuclear counterstain is DAPI (blue).

• IP

Supplier Data

Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (AB251461)

This data was developed using ab206914, the same antibody clone in a different buffer formulation.

BTG4 was immunoprecipitated from 1mg of 293T (Human epithelial cell line from embryonic kidney) transfected with mouse BTG4 with HA-tag whole cell lysate with ab206914 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab206914 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : 293T transfected with mouse BTG4 with HA-tag whole cell lysate, 10μg (Input).
Lane 2 : ab206914 IP in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab206914 in 293T transfected with mouse BTG4 with HA-tag whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-BTG4 antibody [EPRZJU-21] (<a href='/en-us/products/primary-antibodies/btg4-antibody-eprzju-21-ab206914'>ab206914</a>)

Predicted band size: 26 kDa

false

• WB

Supplier Data

Western blot - Anti-BTG4 antibody [EPRZJU-21] - BSA and Azide free (AB251461)

This data was developed using ab206914, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-BTG4 antibody [EPRZJU-21] (<a href='/en-us/products/primary-antibodies/btg4-antibody-eprzju-21-ab206914'>ab206914</a>) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate transfected with empty vector (vector control) at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate lysate transfected with mouse BTG4 with GFP-tag at 20 µg

Lane 3:

Human ovary lysate at 20 µg

Lane 4:

Mouse ovary tissue lysate at 20 µg

Lane 5:

Mouse testis tissue lysate at 20 µg

Lane 6:

Rat ovary tissue lysate at 20 µg

Lane 7:

Rat testis tissue lysate at 20 µg

Lane 8:

Mouse P0 olfactory epithelium lysate at 20 µg

Lane 9:

Rat P0 olfactory epithelium lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 26 kDa

Observed band size: 55 kDa

false

Exposure time: 30s