Skip to main content

Rabbit Recombinant Monoclonal BTK phospho Y551 antibody. Suitable for Flow Cyt, ELISA and reacts with Human, Synthetic peptide - Human samples. Immunogen corresponding to Synthetic Peptide within Human BTK phospho Y551.

Be the first to review this product! Submit a review

Images

Flow Cytometry - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (AB278611), expandable thumbnail
  • ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (AB278611), expandable thumbnail
  • ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (AB278611), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.88% PBS, 0.1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Synthetic Peptide within Human BTK phospho Y551. The exact immunogen used to generate this antibody is proprietary information. Database link Q06187

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytELISA
Human
Tested
Tested
Synthetic peptide - Human
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

0.001-1 µg/mL

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide - Human, Human

Dilution info

-

Notes

-

Target data

Function

Non-receptor tyrosine kinase indispensable for B lymphocyte development, differentiation and signaling (PubMed:19290921). Binding of antigen to the B-cell antigen receptor (BCR) triggers signaling that ultimately leads to B-cell activation (PubMed:19290921). After BCR engagement and activation at the plasma membrane, phosphorylates PLCG2 at several sites, igniting the downstream signaling pathway through calcium mobilization, followed by activation of the protein kinase C (PKC) family members (PubMed:11606584). PLCG2 phosphorylation is performed in close cooperation with the adapter protein B-cell linker protein BLNK (PubMed:11606584). BTK acts as a platform to bring together a diverse array of signaling proteins and is implicated in cytokine receptor signaling pathways (PubMed:16517732, PubMed:17932028). Plays an important role in the function of immune cells of innate as well as adaptive immunity, as a component of the Toll-like receptors (TLR) pathway (PubMed:16517732). The TLR pathway acts as a primary surveillance system for the detection of pathogens and are crucial to the activation of host defense (PubMed:16517732). Especially, is a critical molecule in regulating TLR9 activation in splenic B-cells (PubMed:16517732, PubMed:17932028). Within the TLR pathway, induces tyrosine phosphorylation of TIRAP which leads to TIRAP degradation (PubMed:16415872). BTK also plays a critical role in transcription regulation (PubMed:19290921). Induces the activity of NF-kappa-B, which is involved in regulating the expression of hundreds of genes (PubMed:19290921). BTK is involved on the signaling pathway linking TLR8 and TLR9 to NF-kappa-B (PubMed:19290921). Acts as an activator of NLRP3 inflammasome assembly by mediating phosphorylation of NLRP3 (PubMed:34554188). Transiently phosphorylates transcription factor GTF2I on tyrosine residues in response to BCR (PubMed:9012831). GTF2I then translocates to the nucleus to bind regulatory enhancer elements to modulate gene expression (PubMed:9012831). ARID3A and NFAT are other transcriptional target of BTK (PubMed:16738337). BTK is required for the formation of functional ARID3A DNA-binding complexes (PubMed:16738337). There is however no evidence that BTK itself binds directly to DNA (PubMed:16738337). BTK has a dual role in the regulation of apoptosis (PubMed:9751072).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal BTK phospho Y551 antibody. Suitable for Flow Cyt, ELISA and reacts with Human, Synthetic peptide - Human samples. Immunogen corresponding to Synthetic Peptide within Human BTK phospho Y551.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Synthetic Peptide within Human BTK phospho Y551. The exact immunogen used to generate this antibody is proprietary information. Database link Q06187
Clone number

BtkY551-G12

Purification technique

Affinity purification Protein A/G

Light chain type

kappa

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

BTK or Bruton's tyrosine kinase is an important enzyme in the body that functions mainly in signaling pathways within cells. It is known for its involvement in the development and activation of B cells and is expressed in cells of the hematopoietic system. The BTK protein has a molecular weight of about 76 kDa. Researchers can study BTK using tools like the BTK ELISA kit which helps measure its presence in biological samples. Furthermore there are specific anti-BTK antibodies which assist in the detection of BTK bands during analysis. BTK inhibitors like BTK C481S have been developed to regulate its activity.

Biological function summary

BTK plays an important role in the immune response by transmitting signals from the B cell receptor to the inside of the cell which promotes B cell maturation and survival. It operates as part of a larger signal transduction complex that includes other proteins and molecules. For accurate quantification of BTK expression a BTK sandwich ELISA kit may be utilized capturing and revealing the BTK protein's presence in sample preparations.

Pathways

BTK associates with both the B cell receptor signaling and the PI3K-Akt pathway. These pathways are critical for the proper functioning and proliferation of B cells. BTK interacts with proteins like PLCγ2 and BLNK in the signaling cascade highlighting its central role in transmitting extracellular signals to elicit appropriate cellular responses.

Associated diseases and disorders

BTK is closely linked to X-linked agammaglobulinemia (XLA) a genetic condition marked by an absence of mature B cells. This highlights the connection between BTK function and immune competence. Moreover aberrant BTK activity has been implicated in some types of B cell malignancies such as chronic lymphocytic leukemia (CLL). The BTK pathway's dysregulation in these disorders may involve interactions with proteins like SYK suggesting that targeting BTK could offer therapeutic benefits in managing such conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611), expandable thumbnail

    Flow Cytometry - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)

    Flow cytometric analysis of Ramos cells secondary antibody only negative control (blue) or treated with imatinib (red) or with pervanadate (green) using ab278611 at 0.1 µg/mL.

  • ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611), expandable thumbnail

    ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)

    Direct ELISA using Ramos cellular lysate coated directly to the plate surface after lysis following no treatment or treatment with 1mM pervanadate for 30 min. ELISA wells were tested in duplicate using 0.25 mg/mL total protein coated lysate and 1 ug/mL ab278611.

  • ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611), expandable thumbnail

    ELISA - Anti-BTK (phospho Y551) antibody [BtkY551-G12] (ab278611)

    Peptide ELISA using ab278611 titered starting from 0.3 ug/mL shows binding to only Btk pY551 phospho peptide and no cross-reactivity to Btk Y551 non-phospho peptide or to control peptides.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com