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AB233752

Anti-BTN2A2/BTF2 antibody [4B11C8]

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(2 Publications )

Mouse Monoclonal BTN2A2/BTF2 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BTN2A2 aa 50-250.

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Flow Cytometry - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BTN2A2/BTF2 with ab233752 at 1/200 dilution (green) compared with a negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BTN2A2/BTF2 antibody [4B11C8] (AB233752)

HeLa (human prostate adenocarcinoma cell line) cells stained for BTN2A2/BTF2 (green) using ab233752 at 1/200 dilution in ICC/IF.

Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4B11C8

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human BTN2A2 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q8WVV5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The BTN2A2/BTF2 protein also known as Butyrophilin Subfamily 2 Member A2 plays an important role in immune regulation. It has a molecular mass of approximately 57 kDa. BTN2A2 is part of the butyrophilin family and is expressed mainly in immune-related cells including those found in lymphoid tissues. The protein is a transmembrane glycoprotein with a conserved B30.2 domain which is a characteristic feature of the butyrophilin family.
Biological function summary

BTN2A2/BTF2 modulates T cell activity helping to maintain immune tolerance and preventing overactive immune responses. It functions as part of a larger complex that includes various other immunoregulatory proteins. This complex is critical for fine-tuning immune responses by affecting T cell proliferation and cytokine production. Without proper regulation by BTN2A2 there can be an increased risk of autoimmunity and immune dysregulation.

Pathways

BTN2A2/BTF2 involvement is essential within the immune checkpoint pathways particularly those influencing T cell regulation. It interacts closely with the PD-1 pathway which is significant in mediating immune inhibitory signals. Furthermore BTN2A2 has relationships with other proteins in these pathways such as CTLA-4 which is another key player in maintaining immune balance.

BTN2A2/BTF2 is implicated in autoimmune conditions like rheumatoid arthritis where the immune system attacks healthy tissue. The dysregulation of BTN2A2 might exacerbate these conditions. It also shows associations with inflammatory bowel disease (IBD) where improper immune regulation plays a central role. BTN2A2’s interaction with proteins like PD-1 in these diseases suggests potential therapeutic targets for modulating immune responses and improving patient outcomes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inhibits the proliferation of CD4 and CD8 T-cells activated by anti-CD3 antibodies, T-cell metabolism and IL2 and IFNG secretion.
See full target information BTN2A2

Alternative Names

BT2.2, BTF2, BTN2A2, Butyrophilin subfamily 2 member A2
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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Frontiers in oncology 12:816760 PubMed36033440

2022

Comprehensive Analysis of the Prognostic Value and Immune Infiltration of Butyrophilin Subfamily 2/3 (BTN2/3) Members in Pan-Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Dong He,Zhen Qin,Zihao Liu,Xiaoshuai Ji,Jiajia Gao,Hua Guo,Fan Yang,Haitao Fan,Yanbang Wei,Zixiao Wang,Qian Liu,Qi Pang

Clinical cancer research : an official journal of the American Association for Cancer Research 28:2020-2029 PubMed35165101

2022

Praluzatamab Ravtansine, a CD166-Targeting Antibody-Drug Conjugate, in Patients with Advanced Solid Tumors: An Open-Label Phase I/II Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Valentina Boni,Mary J Fidler,Hendrik-Tobias Arkenau,Alexander Spira,Funda Meric-Bernstam,Nataliya Uboha,Rachel E Sanborn,Randy F Sweis,Patricia LoRusso,Misako Nagasaka,Javier Garcia-Corbacho,Shadia Jalal,James J Harding,Stella K Kim,Iris H C Miedema,Danielle J Vugts,Marc C Huisman,Gerben J C Zwezerijnen,Guus A M S van Dongen,C Willemien Menke van der Houven van Oordt,Song Wang,Tam Dang,Ivan A Zein,Olga Vasiljeva,Susan K Lyman,Virginia Paton,Alison Hannah,Joyce F Liu
View all publications
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Product promise

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