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AB236289

Anti-BTN3A1 antibody

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(1 Publication)

Rabbit Polyclonal BTN3A1 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BTN3A1 aa 1-300.

View Alternative Names

CD277, BTF5, BTN3A1, Butyrophilin subfamily 3 member A1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A1 antibody (AB236289)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A1 antibody (AB236289)

Paraffin-embedded human colon cancer tissue stained for BTN3A1 using ab236289 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-BTN3A1 antibody (AB236289)
  • WB

Supplier Data

Western blot - Anti-BTN3A1 antibody (AB236289)

All lanes:

Western blot - Anti-BTN3A1 antibody (ab236289) at 1/500 dilution

All lanes:

Mouse liver lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/10000 dilution

Predicted band size: 58 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A1 antibody (AB236289)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A1 antibody (AB236289)

Paraffin-embedded human placenta tissue stained for BTN3A1 using ab236289 at 1/100 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human BTN3A1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

O00481

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BTN3A1 also known as CD277 is an immunoregulatory protein with a mass of approximately 57 kDa. It belongs to the butyrophilin family part of the immunoglobulin superfamily. BTN3A1 is expressed in a variety of tissues including the immune system where it is present on the surface of immune cells such as T cells and natural killer cells. The protein plays a role in the immune cell activation and modulation.
Biological function summary

BTN3A1 functions as a co-stimulatory molecule in immune responses. It forms part of a receptor complex that modulates T cell activation and cytokine production. BTN3A1 is essential for the recognition and response to phosphoantigens which are small phosphorylated metabolites. This function allows BTN3A1 to regulate immune responses effectively and shape the body's defense against infections.

Pathways

BTN3A1 plays a critical role in the regulation of the γΔ T cell activation pathway which is important for immune responses against a variety of pathogens. BTN3A1 interacts with other proteins such as BTN3A2 and B7-H3 facilitating the activation and function of γΔ T cells. These interactions influence the immune system's ability to respond appropriately to infections or malignancies.

BTN3A1 has been implicated in autoimmune diseases and cancer. Alterations in BTN3A1 expression or function can contribute to autoimmune disorders due to dysregulated immune responses. Additionally BTN3A1 is studied in the context of cancer where its interactions with proteins like BTN3A3 may influence tumor immune escape mechanisms. Understanding BTN3A1's role in these conditions provides insights into potential therapeutic targets for managing autoimmune diseases and cancer.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in T-cell activation and in the adaptive immune response. Regulates the proliferation of activated T-cells. Regulates the release of cytokines and IFNG by activated T-cells. Mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate.
See full target information BTN3A1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 13:984 PubMed36418890

2022

BTN3A1 promotes tumor progression and radiation resistance in esophageal squamous cell carcinoma by regulating ULK1-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Yang,Bo Cheng,Pengxiang Chen,Xiaozheng Sun,Zhihua Wen,Yufeng Cheng
View all publications
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