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Mouse Monoclonal BTN3A2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BTN3A2.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-BTN3A2 antibody [OTI1A6] (AB118051), expandable thumbnail
  • Western blot - Anti-BTN3A2 antibody [OTI1A6] (AB118051), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (AB118051), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (AB118051), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (AB118051), expandable thumbnail

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human BTN3A2. Database link P78410

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
African green monkey
Expected
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/2000
Notes

-

Expected
Expected

Species
African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
African green monkey
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
African green monkey
Dilution info
1/100
Notes

-

Species
Human
Dilution info
1/100
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Plays a role in T-cell responses in the adaptive immune response. Inhibits the release of IFNG from activated T-cells.

Alternative names

BT3.2, BTF3, BTF4, BTN3A2, Butyrophilin subfamily 3 member A2

Recommended products

Mouse Monoclonal BTN3A2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, African green monkey samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BTN3A2.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human BTN3A2. Database link P78410
Clone number
OTI1A6
Purification technique
Affinity purification
Concentration
Loading...
Purification notes

Purified from cell culture supernatant by affinity chromatography.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

BTN3A2 also known as Butyrophilin Subfamily 3 Member A2 is a protein involved in the immune response. It has a molecular weight of approximately 35 kDa. BTN3A2 expresses in a variety of tissues especially in immune-related cells like T-cells. It forms part of the butyrophilin family known for modulating immune cell functions. The protein is anchored in the cell membrane indicating its role in cell signaling.

Biological function summary

BTN3A2 participates in immune regulation and modulation of T-cell activity. It functions as part of the BTN3A family complex working alongside BTN3A1 and BTN3A3. This protein family influences T-cell receptor signaling and activation contributing to the immune system's ability to respond to pathogens and infections. BTN3A2 plays a role in linking extracellular and intracellular signals that affect immune cell behavior.

Pathways

BTN3A2 integrates within the adaptive immune signaling pathways. One key pathway is the T-cell receptor signaling pathway where BTN3A2 collaborates with BTN3A1 to modulate immune responses. Another significant involvement is in the gamma delta T-cell activation pathway. BTN3A2 partners with proteins like phosphoantigens and other butyrophilins emphasizing its role in controlling T-cell mediated immune functions.

Associated diseases and disorders

BTN3A2 relates to certain autoimmune conditions and cancers. Altered expression of BTN3A2 connects to disorders like psoriasis where dysregulated immune activity plays a part. The protein also connects to chronic lymphocytic leukemia as abnormal BTN3A2 signaling could drive leukemic T-cell behavior. Through these diseases BTN3A2 associates with related proteins such as BTN3A1 and histone deacetylases interacting in pathways disrupted in these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-BTN3A2 antibody [OTI1A6] (ab118051), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BTN3A2 antibody [OTI1A6] (ab118051)

    COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY BTN3A2 stained for BTN3A2 (Green) using ab118051 at 1/100 dilution in ICC/IF.

  • Western blot - Anti-BTN3A2 antibody [OTI1A6] (ab118051), expandable thumbnail

    Western blot - Anti-BTN3A2 antibody [OTI1A6] (ab118051)

    All lanes: Western blot - Anti-BTN3A2 antibody [OTI1A6] (ab118051) at 1/2000 dilution

    Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control at 5 µg

    Lane 2: HEK-293T cell lysate transfected with pCMV6-ENTRY BTN3A2 cDNA at 5 µg

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051)

    Paraffin-embedded Human Breast tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051)

    Paraffin-embedded Human colon tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051)

    Paraffin-embedded Human kidney tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051)

    Paraffin-embedded Human ovary tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051)

    Paraffin-embedded Human lung tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTN3A2 antibody [OTI1A6] (ab118051)

    Paraffin-embedded Human endometrium tissue stained for BTN3A2 using ab118051 at 1/50 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-BTN3A2 antibody [OTI1A6] (ab118051), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-BTN3A2 antibody [OTI1A6] (ab118051)

    HT-29 (Human colorectal adenocarcinoma cell line) cells transiently transfected by pCMV6-ENTRY BTN3A2 stained for BTN3A2 (Green) using ab118051 at 1/100 dilution in ICC/IF.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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