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AB223053

Anti-BTNL2 antibody

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(1 Publication)

Rabbit Polyclonal BTNL2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human BTNL2 aa 1 to C-terminus.

View Alternative Names

Butyrophilin-like protein 2, BTL-II, BTNL2

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTNL2 antibody (AB223053)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTNL2 antibody (AB223053)

Paraffin embedded sections of human testis tissue were stained for BTNL2 with ab223053 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTNL2 antibody (AB223053)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BTNL2 antibody (AB223053)

Paraffin embedded sections of human pancreas tissue were stained for BTNL2 with ab223053 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-BTNL2 antibody (AB223053)
  • WB

Supplier Data

Western blot - Anti-BTNL2 antibody (AB223053)

All lanes:

Western blot - Anti-BTNL2 antibody (ab223053) at 1/2000 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Lane 2:

K562 (human chronic myelogenous leukemia cell line from bone marrow ) whole cell lysate

Lane 3:

Mouse brain lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG at 1/50000 dilution

Predicted band size: 50 kDa

Observed band size: 51 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human BTNL2 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UIR0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
>95% pure
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BTNL2 also known as Butyrophilin-like protein 2 functions as an immunoregulatory protein. This protein with a molecular mass of approximately 55 kDa predominantly expresses in the epithelial cells of the small intestine and thymus. Researchers have identified its expression in certain peripheral tissues indicating a role in the immune response system where it influences T-cell activation and proliferation.
Biological function summary

BTNL2 plays a significant role in modulating immune responses. It forms part of a complex with other immune checkpoint molecules providing inhibitory signals to T cells. This interaction helps maintain immune system balance and prevents excessive immune responses. The presence of BTNL2 in epithelial tissues suggests its involvement in mucosal immunity and interaction with the gut's microbiome.

Pathways

BTNL2 directly participates in the immune checkpoint pathway. It negatively regulates the T-cell receptor signaling pathway in concert with proteins such as CTLA-4 and PD-1. These pathways are critical for maintaining immune homeostasis and avoiding autoimmunity by controlling T-cell activity.

Mutations or polymorphisms in BTNL2 relate to conditions like sarcoidosis and ulcerative colitis. BTNL2's interactions with human leukocyte antigen (HLA) proteins contribute to these associations. The modulation of T-cell activity by BTNL2 may influence the development of immune-mediated diseases making it a target of interest for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of T-cell proliferation.
See full target information BTNL2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials research 29:0244 PubMed40873954

2025

Engineered Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Mitigate Liver Fibrosis by Delivering USP10 to Reprogram Macrophage Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Tian,Xia Zhou,Linhua Zheng,Jingyi Liu,Miao Zhang,Shuoyi Ma,Xiaohong Zheng,Guanya Guo,Ruobing Ju,Fangfang Yang,Yansheng Liu,Bo Li,Yinan Hu,Erzhuo Xia,Rui Su,Keshuai Sun,Lina Cui,Changcun Guo,Xinmin Zhou,Jingbo Wang,Yulong Shang,Ying Han
View all publications
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Product promise

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