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AB250420

Anti-BTNL2 antibody [EPR12252] - BSA and Azide free

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Rabbit Recombinant Monoclonal BTNL2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Butyrophilin-like protein 2, BTL-II, BTNL2

1 Images
Western blot - Anti-BTNL2 antibody [EPR12252] - BSA and Azide free (AB250420)
  • WB

Supplier Data

Western blot - Anti-BTNL2 antibody [EPR12252] - BSA and Azide free (AB250420)

This data was developed using ab181198, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BTNL2 antibody [EPR12252] (<a href='/en-us/products/primary-antibodies/btnl2-antibody-epr12252-ab181198'>ab181198</a>) at 1/2000 dilution

Lane 1:

Human spleen tissue lysate at 20 µg

Lane 2:

Human tonsil tissue lysate at 20 µg

Lane 3:

Human small intestine tissue lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 50 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12252

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab250420 is the carrier-free version of ab181198.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BTNL2 also known as Butyrophilin-like protein 2 functions as an immunoregulatory protein. This protein with a molecular mass of approximately 55 kDa predominantly expresses in the epithelial cells of the small intestine and thymus. Researchers have identified its expression in certain peripheral tissues indicating a role in the immune response system where it influences T-cell activation and proliferation.
Biological function summary

BTNL2 plays a significant role in modulating immune responses. It forms part of a complex with other immune checkpoint molecules providing inhibitory signals to T cells. This interaction helps maintain immune system balance and prevents excessive immune responses. The presence of BTNL2 in epithelial tissues suggests its involvement in mucosal immunity and interaction with the gut’s microbiome.

Pathways

BTNL2 directly participates in the immune checkpoint pathway. It negatively regulates the T-cell receptor signaling pathway in concert with proteins such as CTLA-4 and PD-1. These pathways are critical for maintaining immune homeostasis and avoiding autoimmunity by controlling T-cell activity.

Mutations or polymorphisms in BTNL2 relate to conditions like sarcoidosis and ulcerative colitis. BTNL2's interactions with human leukocyte antigen (HLA) proteins contribute to these associations. The modulation of T-cell activity by BTNL2 may influence the development of immune-mediated diseases making it a target of interest for therapeutic research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Negative regulator of T-cell proliferation.
See full target information BTNL2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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