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AB177473

Anti-BTNL3 antibody [EPR8411(2)(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal BTNL3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BTNLR, COLF4100, UNQ744/PRO1472, BTNL3, Butyrophilin-like protein 3, Butyrophilin-like receptor

2 Images
Flow Cytometry (Intracellular) - Anti-BTNL3 antibody [EPR8411(2)(B)] (AB177473)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BTNL3 antibody [EPR8411(2)(B)] (AB177473)

Intracellular flow cytometric analysis of permeabilized K562 cells labeling BTNL3with ab177473 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (AB177473)
  • WB

Supplier Data

Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (AB177473)

All lanes:

Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (ab177473) at 1/1000 dilution

Lane 1:

Human small intestine lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Lane 3:

Caco2 lysate at 10 µg

Lane 4:

K562 lysate at 10 µg

Predicted band size: 52 kDa

true

  • Carrier free

    Anti-BTNL3 antibody [EPR8411(2)(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8411(2)(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BTNL3 also known as butyrophilin-like 3 is a protein that plays a role in immune system regulation. It has a molecular weight of approximately 55 kDa and is primarily expressed in the epithelial tissues including the skin and gut. BTNL3 belongs to the butyrophilin family which is part of the immunoglobulin superfamily. This protein is recognized for its function in modulating immune responses by interacting with T cells through its extracellular domains.
Biological function summary

BTNL3 influences immune cell activity by acting as a modulator affecting the activation and suppression of T cells. BTNL3 can form a complex with other butyrophilin-like proteins such as BTNL8 to exert its regulatory effects. Its expression in epithelial tissues suggests a role in maintaining epithelial barrier integrity and immune homeostasis at these sites.

Pathways

BTNL3 takes part in the regulation of T cell receptor signaling. It contributes to the broader immunological pathways including the adaptive immune response. Other proteins like BTN3A1 interact within these pathways to modulate immune cell function and ensure balanced immune responses highlighting the coordinated actions required for proper immune surveillance.

BTNL3 has connections to inflammatory bowel disease and skin disorders. Altered expression or function of BTNL3 may disrupt the immune equilibrium contributing to these conditions. Furthermore it intersects with proteins such as BTNL8 which may also be implicated in these pathological states indicating the importance of BTNL3 in maintaining immune health and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information BTNL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Science signaling 12: PubMed30755478

2019

An immunoproteomic approach to characterize the CAR interactome and signalosome.

Applications

Unspecified application

Species

Unspecified reactive species

Maria C Ramello,Ismahène Benzaïd,Brent M Kuenzi,Maritza Lienlaf-Moreno,Wendy M Kandell,Daniel N Santiago,Mibel Pabón-Saldaña,Lancia Darville,Bin Fang,Uwe Rix,Sean Yoder,Anders Berglund,John M Koomen,Eric B Haura,Daniel Abate-Daga
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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