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AB249978

Anti-BTNL3 antibody [EPR8411(2)(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal BTNL3 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BTNLR, COLF4100, UNQ744/PRO1472, BTNL3, Butyrophilin-like protein 3, Butyrophilin-like receptor

2 Images
Flow Cytometry (Intracellular) - Anti-BTNL3 antibody [EPR8411(2)(B)] - BSA and Azide free (AB249978)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-BTNL3 antibody [EPR8411(2)(B)] - BSA and Azide free (AB249978)

This data was developed using ab177473, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized K562 cells labeling BTNL3 with ab177473 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] - BSA and Azide free (AB249978)
  • WB

Supplier Data

Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] - BSA and Azide free (AB249978)

This data was developed using ab177473, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-BTNL3 antibody [EPR8411(2)(B)] (<a href='/en-us/products/primary-antibodies/btnl3-antibody-epr84112b-ab177473'>ab177473</a>) at 1/1000 dilution

Lane 1:

Human small intestine lysate at 10 µg

Lane 2:

Jurkat lysate at 10 µg

Lane 3:

Caco2 lysate at 10 µg

Lane 4:

K562 lysate at 10 µg

Predicted band size: 52 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8411(2)(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab249978 is the carrier-free version of ab177473.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BTNL3 also known as butyrophilin-like 3 is a protein that plays a role in immune system regulation. It has a molecular weight of approximately 55 kDa and is primarily expressed in the epithelial tissues including the skin and gut. BTNL3 belongs to the butyrophilin family which is part of the immunoglobulin superfamily. This protein is recognized for its function in modulating immune responses by interacting with T cells through its extracellular domains.
Biological function summary

BTNL3 influences immune cell activity by acting as a modulator affecting the activation and suppression of T cells. BTNL3 can form a complex with other butyrophilin-like proteins such as BTNL8 to exert its regulatory effects. Its expression in epithelial tissues suggests a role in maintaining epithelial barrier integrity and immune homeostasis at these sites.

Pathways

BTNL3 takes part in the regulation of T cell receptor signaling. It contributes to the broader immunological pathways including the adaptive immune response. Other proteins like BTN3A1 interact within these pathways to modulate immune cell function and ensure balanced immune responses highlighting the coordinated actions required for proper immune surveillance.

BTNL3 has connections to inflammatory bowel disease and skin disorders. Altered expression or function of BTNL3 may disrupt the immune equilibrium contributing to these conditions. Furthermore it intersects with proteins such as BTNL8 which may also be implicated in these pathological states indicating the importance of BTNL3 in maintaining immune health and its potential as a therapeutic target.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information BTNL3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong
View all publications
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Product promise

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