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AB227228

Anti-Bub1 antibody

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(2 Publications)

Rabbit Polyclonal BUB1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BUB1.

View Alternative Names

BUB1L, BUB1, Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1A

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Bub1 antibody (AB227228)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Bub1 antibody (AB227228)

Paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for Bub1 (green) using ab227228 at 1/500 dilution in ICC/IF. Counterstain : Alpha Tubulin filaments were labeled with an anti-alpha Tubulin antibody (red).

Western blot - Anti-Bub1 antibody (AB227228)
  • WB

Supplier Data

Western blot - Anti-Bub1 antibody (AB227228)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Bub1 antibody (ab227228) at 1/1000 dilution

All lanes:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Predicted band size: 122 kDa

false

Western blot - Anti-Bub1 antibody (AB227228)
  • WB

Supplier Data

Western blot - Anti-Bub1 antibody (AB227228)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Bub1 antibody (ab227228) at 1/3000 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 122 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human BUB1. The exact immunogen used to generate this antibody is proprietary information.

O43683

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bub1 protein often referred to as 'Bub1' plays a mechanical role in cell cycle regulation especially during mitosis. It is known by other names such as BUB1 mitotic checkpoint serine/threonine kinase. Bub1 has a molecular mass of approximately 120 kDa. This protein is expressed in various tissues with higher levels in rapidly dividing cells. The location of its action is mainly the nucleus where it associates with kinetochores during cell division functioning in the spindle assembly checkpoint.
Biological function summary

Bub1 protein acts as a serine/threonine-protein kinase that is essential for proper chromosome segregation. It forms part of the spindle checkpoint complex along with other proteins such as BubR1 and Mad2. This complex ensures that chromosomes are correctly attached to the spindle microtubules before anaphase begins. Bub1's kinase activity contributes to the prevention of premature anaphase onset thereby helping maintain genomic stability.

Pathways

Bub1 is significantly involved in the mitotic checkpoint pathway and the cell cycle control pathway. Within these pathways Bub1 interacts closely with proteins like CDC20 and APC/C which are integral parts of the anaphase-promoting complex. Bub1 modifies the activity of these proteins to delay anaphase onset until all chromosomes are properly aligned ensuring the fidelity of cell division.

Bub1 protein shows a connection to various cancer types notably breast cancer and colorectal cancer. Defects or overexpression in Bub1 can disrupt the spindle assembly checkpoint leading to chromosome missegregation and aneuploidy hallmarks of cancerous cells. Bub1 is also linked to other spindle checkpoint proteins such as Mad1 and Bub3. Malfunction in any of these proteins including Bub1 can contribute to tumorigenesis due to impaired cell cycle control.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis : it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Promotes the centromeric localization of TOP2A (PubMed : 35044816). Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.
See full target information BUB1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

In vivo (Athens, Greece) 37:2421-2432 PubMed37905617

2023

Chemical Carcinogen (Dimethyl-benzanthracene) Induced Transplantable Cancer in Fanconi Anemia (Fanca-/-) Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Michael W Epperly,Amitava Mukherjee,Renee Fisher,Donna Shields,Wen Hou,Hong Wang,Lora H Rigatti,Anthony Green,M Saiful Huq,Joel S Greenberger

Molecular cell 82:4018-4032.e9 PubMed36332605

2022

LncRNA CCTT-mediated RNA-DNA and RNA-protein interactions facilitate the recruitment of CENP-C to centromeric DNA during kinetochore assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zhang,Dongpeng Wang,Yajing Hao,Shuheng Wu,Jianjun Luo,Yuanchao Xue,Di Wang,Guohong Li,Lihui Liu,Changwei Shao,Huiyan Li,Jinfeng Yuan,Maoxiang Zhu,Xiang-Dong Fu,Xiao Yang,Runsheng Chen,Yan Teng
View all publications
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Product promise

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