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Rabbit Recombinant Monoclonal BUB1 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

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Images

Western blot - Anti-Bub1 antibody [EPR18947] (AB195268), expandable thumbnail
  • Western blot - Anti-Bub1 antibody [EPR18947] (AB195268), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Bub1 antibody [EPR18947] (AB195268), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub1 antibody [EPR18947] (AB195268), expandable thumbnail
  • Western blot - Anti-Bub1 antibody [EPR18947] (AB195268), expandable thumbnail

Publications

  • iScience 26:1076812023
    Inhibition of the serine/threonine kinase BUB1 reverses taxane resistance in prostate cancer.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Maria J Martinez,Rolando D Z Lyles,Nahuel Peinetti,Alex M Grunfeld,Kerry L Burnstein
    PubMed 37705955
  • Ecotoxicology and environmental safety 256:1148232023
    miR-186 induces tetraploidy in arsenic exposed human keratinocytes.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Ana P Ferragut Cardoso,Alexandra N Nail,Mayukh Banerjee,Sandra S Wise,J Christopher States
    PubMed 36989553

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Tested
Tested
Expected
Mouse
Expected
Tested
Tested
Rat
Expected
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

-

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

Our in house IHC testing showed positive staining in testis tissue ONLY. Other tissues were negative. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/50

Notes

Our in house IHC testing showed positive staining in testis tissue ONLY. Other tissues were negative. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

    1 products for Alternative Version

      Target data

      Function

      Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Required for centromeric enrichment of AUKRB in prometaphase. Plays an important role in defining SGO1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.

      Alternative names

      Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1A, BUB1, BUB1L

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      Rabbit Recombinant Monoclonal BUB1 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 10 publications.

      Alternative names

      Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1A, BUB1, BUB1L

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR18947

      Purification technique

      Affinity purification Protein A

      Specificity

      ab195268 shows stronger signal in mouse and rat testis tissues but weaker in the human testis.

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our in house IHC testing showed positive staining in testis tissue ONLY. Other tissues were negative.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      8 product images

      • Western blot - Anti-Bub1 antibody [EPR18947] (ab195268), expandable thumbnail

        Western blot - Anti-Bub1 antibody [EPR18947] (ab195268)

        Lanes 1- 2: Merged signal (red and green). Green - ab195268 observed at 125 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) observed at 37 kDa.

        ab195268 was shown to react with Bub1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human BUB1 knockout HeLa cell line ab265145 (knockout cell lysate Human BUB1 knockout HeLa cell lysate ab257373) was used. Wild-type HeLa and BUB1 knockout HeLa cell lysates were subjected to SDS-PAGE. ab195268 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-Bub1 antibody [EPR18947] (ab195268) at 1/1000 dilution

        Lane 1: Wild-type HeLa cell lysate at 20 µg

        Lane 2: BUB1 knockout HeLa cell lysate at 20 µg

        Performed under reducing conditions.

        Predicted band size: 122 kDa

        Observed band size: 125 kDa

      • Western blot - Anti-Bub1 antibody [EPR18947] (ab195268), expandable thumbnail

        Western blot - Anti-Bub1 antibody [EPR18947] (ab195268)

        Lane 1: Wild-type HAP1 cell lysate (20 μg)
        Lane 2: Bub1 knockout HAP1 cell lysate (20 μg)
        Lane 3: HeLa cell lysate (20 μg)
        Lane 4: F9 cell lysate (20 μg)
        Lanes 1 - 4: Merged signal (red and green). Green - ab195268 observed at 125 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

        ab195268 was shown to recognize Bub1 when Bub1 knockout samples were used, along with additional cross-reactive bands. Wild-type and Bub1 knockout samples were subjected to SDS-PAGE. ab195268 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ( Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

        All lanes: Western blot - Anti-Bub1 antibody [EPR18947] (ab195268)

        Predicted band size: 122 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-Bub1 antibody [EPR18947] (ab195268), expandable thumbnail

        Immunocytochemistry/ Immunofluorescence - Anti-Bub1 antibody [EPR18947] (ab195268)

        ab195268 staining Bub1 in HeLa (human cervix adenocarcinoma epithelial cell) cells by Immunocytochemistry. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% TritonX-100. Samples were incubated with primary antibody at 1:100 dilution (7.8 μg/ml). Alexa Fluor® 594 Anti-alpha tubulin [DM1A] – Microtubule Marker (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used as the counterstain antibody at 1:200 dilution (2.5 μg/ml). An AlexaFluor®488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as a secondary antibody at 1:1000 dilution (2μg/ml). DAPI was used as a nuclear counterstain. Confocal image showing positive kinetochore staining in HeLa cells in M phase.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub1 antibody [EPR18947] (ab195268), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub1 antibody [EPR18947] (ab195268)

        Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Bub1 with ab195268 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

        Nucleus staining on Rat testis is observed.

        Counter stained with Hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Western blot - Anti-Bub1 antibody [EPR18947] (ab195268), expandable thumbnail

        Western blot - Anti-Bub1 antibody [EPR18947] (ab195268)

        Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

        Exposure time: Lane 1 & 2: 10 seconds; Lane 2: 3 minutes.

        All lanes: Western blot - Anti-Bub1 antibody [EPR18947] (ab195268) at 1/1000 dilution

        Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

        Lane 2: K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate at 20 µg

        Lane 3: U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Predicted band size: 122 kDa

        Observed band size: 122 kDa

      • Western blot - Anti-Bub1 antibody [EPR18947] (ab195268), expandable thumbnail

        Western blot - Anti-Bub1 antibody [EPR18947] (ab195268)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        All lanes: Western blot - Anti-Bub1 antibody [EPR18947] (ab195268) at 1/1000 dilution

        Lane 1: Human testis lysate at 10 µg

        Lane 2: Human fetal liver lysate at 10 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

        Predicted band size: 122 kDa

        Observed band size: 122 kDa

        Exposure time: 3min

      • Western blot - Anti-Bub1 antibody [EPR18947] (ab195268), expandable thumbnail

        Western blot - Anti-Bub1 antibody [EPR18947] (ab195268)

        Blocking/Dilution buffer: 5% NFDM/TBST.

        The molecular weight observed is consistent with what has been described in the literature PMID:17189386
        PMID:16864798

        All lanes: Western blot - Anti-Bub1 antibody [EPR18947] (ab195268) at 1/1000 dilution

        Lane 1: Mouse testis lysate at 20 µg

        Lane 2: Rat testis lysate at 20 µg

        Lane 3: F9 (Mouse embryonic testicular cancer cell line) whole cell lysate at 20 µg

        Lane 4: mESC (Mouse embryonic stem cell line) whole cell lysate at 20 µg

        Secondary

        All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

        Predicted band size: 122 kDa

        Observed band size: 122 kDa

        Exposure time: 3min

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub1 antibody [EPR18947] (ab195268), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub1 antibody [EPR18947] (ab195268)

        Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Bub1 with ab195268 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

        Nucleus staining on Mouse testis is observed.

        Counter stained with Hematoxylin.

        Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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