Rabbit Polyclonal BUB3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Mitotic checkpoint protein BUB3 aa 1-50.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Mitotic checkpoint protein BUB3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O43684
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/15000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
Alternative names
Mitotic checkpoint protein BUB3, BUB3
Recommended products
Rabbit Polyclonal BUB3 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Mitotic checkpoint protein BUB3 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Mitotic checkpoint protein BUB3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link O43684
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bub3 also known as budding uninhibited by benzimidazoles-3 is an important component of the spindle checkpoint machinery. Weighing approximately 35 kDa this protein is expressed ubiquitously in eukaryotic cells. Bub3 interacts with other spindle checkpoint proteins and takes part in chromosome segregation during mitosis. It primarily functions in the nucleus during the cell cycle ensuring proper attachment of chromosomes to the mitotic spindle which is important for accurate cell division.
- Biological function summary
Bub3 participates in the mitotic checkpoint complex an essential mediator of cell cycle arrest in response to improper spindle attachment. This complex includes Bub1 BubR1 and Mad3 where Bub3 acts as an adapter facilitating interactions within the complex. It helps maintain chromosomal stability by preventing premature anaphase onset therefore ensuring that damaged or unaligned chromosomes do not progress through cell division. By doing so Bub3 safeguards against aneuploidy and genomic instability.
- Pathways
Bub3 is linked to the spindle assembly checkpoint (SAC) and the cell cycle regulation pathway. In SAC Bub3 collaborates closely with proteins such as Bub1 and Mad1 to inhibit the anaphase-promoting complex/cyclosome (APC/C) until all chromosomes are properly aligned. The interplay between Bub3 and BubR1 is important for delaying the chief exit phases of mitosis ensuring that all cellular conditions are favorable for division. These interactions prevent errors that might cause irreparable damage or cell death.
- Associated diseases and disorders
Bub3 has associations with cancer and some neurodegenerative diseases. Its dysregulation can lead to aneuploidy a hallmark of many cancers through faulty chromosome segregation. In several cancer types altered expression of Bub3 and its partners such as Bub1 and Mad2 has been documented. Similarly emerging studies suggest links between Bub3 malfunction and neurodegeneration where alterations in cell division and genomic stability may contribute to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Bub3 antibody (ab264121)
4-20% SDS-PAGE.
All lanes: Western blot - Anti-Bub3 antibody (ab264121) at 0.066 µg/mL
Lane 1: Mock treated HeLa whole cell lysate at 50 µg
Lane 2: HeLa cell lysate treated with Nocodazole at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub3 antibody (ab264121)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Bub3 using ab264121 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB
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