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AB133699

Anti-Bub3 antibody [EPR5319(2)]

3

(1 Review)

|

(12 Publications)

Rabbit Recombinant Monoclonal BUB3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 12 publications.

View Alternative Names

Mitotic checkpoint protein BUB3, BUB3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub3 antibody [EPR5319(2)] (AB133699)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub3 antibody [EPR5319(2)] (AB133699)

Immunohistochemical analysis of paraffin-embedded human breast tissue labelling Bub3 using ab133699 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Bub3 antibody [EPR5319(2)] (AB133699)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bub3 antibody [EPR5319(2)] (AB133699)

Immunofluorescent staining Bub3 in 293T cells using ab133699 at 1/250 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub3 antibody [EPR5319(2)] (AB133699)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bub3 antibody [EPR5319(2)] (AB133699)

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labelling Bub3 using ab133699 at 1/100 dilution

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Bub3 antibody [EPR5319(2)] (AB133699)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Bub3 antibody [EPR5319(2)] (AB133699)

Immunocytochemistry/Immunofluorescence analysis of HepG2 (human hepatocellular carcinoma) labelling Bub3 with purified ab133699 at 1/2000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (ab150077). Nuclei counterstained with DAPI (blue).

Control : PBS only

Western blot - Anti-Bub3 antibody [EPR5319(2)] (AB133699)
  • WB

Unknown

Western blot - Anti-Bub3 antibody [EPR5319(2)] (AB133699)

All lanes:

Western blot - Anti-Bub3 antibody [EPR5319(2)] (ab133699) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

Observed band size: 40 kDa

false

  • Carrier free

    Anti-Bub3 antibody [EPR5319(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5319(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/2000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bub3 also known as budding uninhibited by benzimidazoles-3 is an important component of the spindle checkpoint machinery. Weighing approximately 35 kDa this protein is expressed ubiquitously in eukaryotic cells. Bub3 interacts with other spindle checkpoint proteins and takes part in chromosome segregation during mitosis. It primarily functions in the nucleus during the cell cycle ensuring proper attachment of chromosomes to the mitotic spindle which is important for accurate cell division.
Biological function summary

Bub3 participates in the mitotic checkpoint complex an essential mediator of cell cycle arrest in response to improper spindle attachment. This complex includes Bub1 BubR1 and Mad3 where Bub3 acts as an adapter facilitating interactions within the complex. It helps maintain chromosomal stability by preventing premature anaphase onset therefore ensuring that damaged or unaligned chromosomes do not progress through cell division. By doing so Bub3 safeguards against aneuploidy and genomic instability.

Pathways

Bub3 is linked to the spindle assembly checkpoint (SAC) and the cell cycle regulation pathway. In SAC Bub3 collaborates closely with proteins such as Bub1 and Mad1 to inhibit the anaphase-promoting complex/cyclosome (APC/C) until all chromosomes are properly aligned. The interplay between Bub3 and BubR1 is important for delaying the chief exit phases of mitosis ensuring that all cellular conditions are favorable for division. These interactions prevent errors that might cause irreparable damage or cell death.

Bub3 has associations with cancer and some neurodegenerative diseases. Its dysregulation can lead to aneuploidy a hallmark of many cancers through faulty chromosome segregation. In several cancer types altered expression of Bub3 and its partners such as Bub1 and Mad2 has been documented. Similarly emerging studies suggest links between Bub3 malfunction and neurodegeneration where alterations in cell division and genomic stability may contribute to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
See full target information Mitotic checkpoint protein BUB3
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 12: PubMed36428310

2022

BUBR1 as a Prognostic Biomarker in Canine Oral Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Leonor Delgado,Luís Monteiro,Patrícia Silva,Hassan Bousbaa,Fernanda Garcez,João Silva,Paula Brilhante-Simões,Isabel Pires,Justina Prada

Scientific reports 12:13160 PubMed35915203

2022

The E3 ubiquitin ligase HECTD1 contributes to cell proliferation through an effect on mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Natalie Vaughan,Nico Scholz,Catherine Lindon,Julien D F Licchesi

Frontiers in oncology 11:752933 PubMed34804941

2021

Mitotic Errors Promote Genomic Instability and Leukemia in a Novel Mouse Model of Fanconi Anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Donna M Edwards,Dana K Mitchell,Zahi Abdul-Sater,Ka-Kui Chan,Zejin Sun,Aditya Sheth,Ying He,Li Jiang,Jin Yuan,Richa Sharma,Magdalena Czader,Pei-Ju Chin,Yie Liu,Guillermo de Cárcer,Grzegorz Nalepa,Hal E Broxmeyer,D Wade Clapp,Elizabeth A Sierra Potchanant

Frontiers in cell and developmental biology 9:671685 PubMed34277613

2021

Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic Oocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Yong Zhang,Jian Li,Ying-Chun Ouyang,Tie-Gang Meng,Chun-Hui Zhang,Wei Yue,Qing-Yuan Sun,Wei-Ping Qian

Reproductive biology and endocrinology : RB&E 19:57 PubMed33874950

2021

WAPL orchestrates porcine oocyte meiotic progression via control of spindle assembly checkpoint activity.

Applications

Unspecified application

Species

Unspecified reactive species

Changyin Zhou,Yilong Miao,Xue Zhang,Bo Xiong

Science advances 7: PubMed33827814

2021

Posttranslational regulation of FOXA1 by Polycomb and BUB3/USP7 deubiquitin complex in prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Su H Park,Ka-Wing Fong,Jung Kim,Fang Wang,Xiaodong Lu,Yongik Lee,Lourdes T Brea,Kristine Wadosky,Chunming Guo,Sarki A Abdulkadir,John D Crispino,Deyu Fang,Panagiotis Ntziachristos,Xin Liu,Xue Li,Yong Wan,David W Goodrich,Jonathan C Zhao,Jindan Yu

Oncogene 40:1821-1835 PubMed33564066

2021

The long noncoding RNA CRYBG3 induces aneuploidy by interfering with spindle assembly checkpoint via direct binding with Bub3.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyang Guo,Yingchu Dai,Wentao Hu,Yongsheng Zhang,Zhifei Cao,Weiwei Pei,Ningang Liu,Jing Nie,Anqing Wu,Weidong Mao,Lei Chang,Bingyan Li,Hailong Pei,Tom K Hei,Guangming Zhou

Science advances 6:eaax3969 PubMed32284991

2020

The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I.

Applications

Unspecified application

Species

Unspecified reactive species

Changyin Zhou,Yilong Miao,Zhaokang Cui,Xiayan ShiYang,Yu Zhang,Bo Xiong

Nature communications 11:24 PubMed31911655

2020

The RepID-CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Min Jang,Jenny F Nathans,Haiqing Fu,Christophe E Redon,Lisa M Jenkins,Bhushan L Thakur,Lőrinc S Pongor,Adrian M Baris,Jacob M Gross,Maura J OʹNeill,Fred E Indig,Steven D Cappell,Mirit I Aladjem

Cell cycle (Georgetown, Tex.) 18:1254-1267 PubMed31111756

2019

Inhibition of neddylation causes meiotic arrest in mouse oocyte.

Applications

Unspecified application

Species

Unspecified reactive species

Mo Yang,Yimei Jin,Siying Fan,Xiaoling Liang,Jialin Jia,Zhongzhou Tan,Tao Huang,Yuan Li,Teng Ma,Mo Li
View all publications
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