AB54894

Anti-BubR1 antibody [2G5]

Name: Anti-BubR1 antibody [2G5] (ab54894) | Abcam
Brand: Abcam Limited
SKU: AB54894
Price: 715 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(14 Publications)

Mouse Monoclonal BubR1 antibody. Suitable for Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BUB1B aa 1-150.

View Alternative Names

BUBR1, MAD3L, SSK1, BUB1B, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MAD3/BUB1-related protein kinase, Mitotic checkpoint kinase MAD3L, Protein SSK1, hBUBR1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [2G5] (AB54894)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [2G5] (AB54894)

BubR1 antibody (ab54894) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human spleen.

This image was generated using the ascites version of the product.

Flow Cytometry - Anti-BubR1 antibody [2G5] (AB54894)

Flow Cyt

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Flow Cytometry - Anti-BubR1 antibody [2G5] (AB54894)

Overlay histogram showing HeLa cells stained with ab54894 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54894, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.

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Western blot - Anti-BubR1 antibody [2G5] (AB54894)

BubR1 antibody (ab54894) at 1ug/lane + HeLa cell lysate at 25ug/lane.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-BubR1 antibody [2G5] (ab54894)

Predicted band size: 119 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2G5

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human BUB1B aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O60566

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BubR1 also known as BUB1B is an important protein in the mitotic checkpoint complex with an approximate mass of 120 kDa. BubR1 ensures proper chromosome alignment and segregation during cell division by inhibiting the anaphase-promoting complex/cyclosome (APC/C) until all chromosomes achieve proper attachment to the spindle. This protein localizes mainly to the kinetochores of chromosomes and is commonly expressed in dividing cells throughout the body.

Biological function summary

The spindle assembly checkpoint mechanism involves a complex in which BubR1 plays a central role. BubR1 along with other checkpoint proteins such as Bub1 and Mad2 forms a surveillance complex ensuring the correct attachment of microtubules to kinetochores. This action prevents premature progression to anaphase reducing the risk of aneuploidy and maintaining genomic stability.

Pathways

Mitotic checkpoint control critically involves BubR1 linking closely with several proteins including Mad2 and Cdc20. BubR1 integrates into the spindle checkpoint pathway a central player in regulating mitosis. Within this pathway BubR1 inhibits APC/CCdc20 activity preventing premature anaphase onset until all chromosomes are correctly oriented to divide. Interactions between these proteins ensure accurate mitotic progression and cellular replication fidelity.

BubR1 function directly impacts cancer development and premature aging syndromes. Defective BubR1 activity can lead to chromosomal instability a characteristic of many cancers including colorectal cancer. Moreover reduced BubR1 expression links with premature aging syndromes such as mosaic variegated aneuploidy through its interaction with proteins such as p53 highlighting its role in genome integrity maintenance and cellular aging processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

See full target information BUB1B

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 41:101858 PubMed38242006

2024

mA demethylase ALKBH5 maintains stemness of intrahepatic cholangiocarcinoma by sustaining BUB1B expression and cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Gao,Miao Yu,Zengyuan Liu,Yi Liu,Zhijun Kong,Chunfu Zhu,Xihu Qin,Yan Li,Liming Tang

Journal of cellular physiology 238:2535-2545 PubMed37642322

2023

Maternal SMC2 is essential for embryonic development via participating chromosome condensation in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Xu,Jing-Yi Qiao,Bin-Wang Zhao,Ming-Zhe Dong,Wen-Long Lei,Yuan-Yuan Li,Zhenyu Ju,Heide Schatten,Zhen-Bo Wang,Kui Liu,Qing-Yuan Sun

Nature aging 3:185-201 PubMed37118121

2023

Unique progerin C-terminal peptide ameliorates Hutchinson-Gilford progeria syndrome phenotype by rescuing BUBR1.

Applications

Unspecified application

Species

Unspecified reactive species

Na Zhang,Qianying Hu,Tingting Sui,Lu Fu,Xinglin Zhang,Yu Wang,Xiaojuan Zhu,Baiqu Huang,Jun Lu,Zhanjun Li,Yu Zhang

Journal of cell science 135: PubMed34878135

2022

The APC/C targets the Cep152-Cep63 complex at the centrosome to regulate mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Tischer,Jing Yang,David Barford

Frontiers in oncology 11:752933 PubMed34804941

2021

Mitotic Errors Promote Genomic Instability and Leukemia in a Novel Mouse Model of Fanconi Anemia.

Applications

Unspecified application

Species

Unspecified reactive species

Donna M Edwards,Dana K Mitchell,Zahi Abdul-Sater,Ka-Kui Chan,Zejin Sun,Aditya Sheth,Ying He,Li Jiang,Jin Yuan,Richa Sharma,Magdalena Czader,Pei-Ju Chin,Yie Liu,Guillermo de Cárcer,Grzegorz Nalepa,Hal E Broxmeyer,D Wade Clapp,Elizabeth A Sierra Potchanant

International journal of molecular sciences 21: PubMed32764382

2020

Discovery of Novel Agents on Spindle Assembly Checkpoint to Sensitize Vinorelbine-Induced Mitotic Cell Death Against Human Non-Small Cell Lung Cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Ching Chang,Yu-Ling Tseng,Wohn-Jenn Leu,Chi-Min Du,Yi-Huei Jiang,Lih-Ching Hsu,Jui-Ling Hsu,Duen-Ren Hou,Jih-Hwa Guh

Nature communications 11:24 PubMed31911655

2020

The RepID-CRL4 ubiquitin ligase complex regulates metaphase to anaphase transition via BUB3 degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Min Jang,Jenny F Nathans,Haiqing Fu,Christophe E Redon,Lisa M Jenkins,Bhushan L Thakur,Lőrinc S Pongor,Adrian M Baris,Jacob M Gross,Maura J OʹNeill,Fred E Indig,Steven D Cappell,Mirit I Aladjem

Cell death and differentiation 26:2046-2060 PubMed30742091

2019

Cell cycle arrest in mitosis promotes interferon-induced necroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Tanja Frank,Marcel Tuppi,Manuela Hugle,Volker Dötsch,Sjoerd J L van Wijk,Simone Fulda

Scientific reports 6:29735 PubMed27405720

2016

TRAIP is involved in chromosome alignment and SAC regulation in mouse oocyte meiosis.

Applications

Unspecified application

Species

Mouse

Yi-Feng Yuan,Yi-Xin Ren,Peng Yuan,Li-Ying Yan,Jie Qiao

Cell cycle (Georgetown, Tex.) 15:225-33 PubMed26652909

2015

Mitotic phosphatase activity is required for MCC maintenance during the spindle checkpoint.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen M Foss,Alexander C Robeson,Sally Kornbluth,Liguo Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BubR1 antibody [2G5]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody [2G5] (AB54894)

Flow Cytometry - Anti-BubR1 antibody [2G5] (AB54894)

Western blot - Anti-BubR1 antibody [2G5] (AB54894)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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