Anti-BubR1 antibody

• IHC

CiteAb

Immunohistochemistry - Anti-BubR1 antibody (AB28193)

Immunohistochemistry-immunofluorescence using Anti-BubR1 antibody, ab28193. Publication image from Li, H. et al., 2020, Adv Sci (Weinh), 32714760. Legend direct from paper.

Defective progression of meiosis to metaphase I (MI) by D166G‐Dpagt1 mutant oocytes. A) IF staining of spindles (green) and chromosomes (blue) in oocytes matured in vitro for 8 h (top panel). Quantification of the rate of oocytes with various meiotic behaviors is shown in the bottom panel. B) Assessment of the active status of SAC by IF staining of BUBR1 (green) and kinetochore (red) in oocytes (top panel). Normal WT oocytes matured in vitro for 3 h serve as positive control for active SAC. Quantification of the percentage of oocytes with active SAC is shown in the bottom bar graph. C) Analysis of kinetochore–microtubule attachment in oocytes matured in vitro for 8 h. Microtubules are stained in green, kinetochores are stained in red, and chromosomes are stained blue. Quantification of the percentage of oocytes with misattached kinetochore–microtubules are shown in the bottom panel. Arrows indicate chromosomes with no microtubule attached. Scale bars indicate 5 µm. *p < 0.05, compared to WTs by Student's t‐test.