AB70544

Anti-BubR1 antibody

Name: Anti-BubR1 antibody (ab70544) | Abcam
Brand: Abcam Limited
SKU: AB70544
Price: 545 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal BubR1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BUB1B.

View Alternative Names

BUBR1, MAD3L, SSK1, BUB1B, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, MAD3/BUB1-related protein kinase, Mitotic checkpoint kinase MAD3L, Protein SSK1, hBUBR1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody (AB70544)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody (AB70544)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling BubR1 with ab70544 at 1/200 (1µg/ml). Detection : DAB.

Unknown

Immunoprecipitation - Anti-BubR1 antibody (AB70544)

1mg whole cell lysate from HeLa cells was immunoprecipitated with ab70544 at 3ug/mg of lysate (lane 1) or a with a control rabbit IgG (lane 2). For the subsequent western blot, 20% of the immunoprecipitae was loaded per lane, and ab70544 was used at 1ug/ml. Detection : chemiluminescence with exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-BubR1 antibody (ab70544)

Predicted band size: 119 kDa

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Western blot - Anti-BubR1 antibody (AB70544)

All lanes:

Western blot - Anti-BubR1 antibody (ab70544) at 0.04 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 119 kDa

Observed band size: 120 kDa

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Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

Synthetic Peptide within Human BUB1B. The exact immunogen used to generate this antibody is proprietary information.

O60566

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BubR1 also known as BUB1B is an important protein in the mitotic checkpoint complex with an approximate mass of 120 kDa. BubR1 ensures proper chromosome alignment and segregation during cell division by inhibiting the anaphase-promoting complex/cyclosome (APC/C) until all chromosomes achieve proper attachment to the spindle. This protein localizes mainly to the kinetochores of chromosomes and is commonly expressed in dividing cells throughout the body.

Biological function summary

The spindle assembly checkpoint mechanism involves a complex in which BubR1 plays a central role. BubR1 along with other checkpoint proteins such as Bub1 and Mad2 forms a surveillance complex ensuring the correct attachment of microtubules to kinetochores. This action prevents premature progression to anaphase reducing the risk of aneuploidy and maintaining genomic stability.

Pathways

Mitotic checkpoint control critically involves BubR1 linking closely with several proteins including Mad2 and Cdc20. BubR1 integrates into the spindle checkpoint pathway a central player in regulating mitosis. Within this pathway BubR1 inhibits APC/CCdc20 activity preventing premature anaphase onset until all chromosomes are correctly oriented to divide. Interactions between these proteins ensure accurate mitotic progression and cellular replication fidelity.

BubR1 function directly impacts cancer development and premature aging syndromes. Defective BubR1 activity can lead to chromosomal instability a characteristic of many cancers including colorectal cancer. Moreover reduced BubR1 expression links with premature aging syndromes such as mosaic variegated aneuploidy through its interaction with proteins such as p53 highlighting its role in genome integrity maintenance and cellular aging processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

See full target information BUB1B

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 31:74 PubMed39014450

2024

BUB1B monoallelic germline variants contribute to prostate cancer predisposition by triggering chromosomal instability.

Applications

Unspecified application

Species

Unspecified reactive species

Maria P Silva,Luísa T Ferreira,Natércia F Brás,Lurdes Torres,Andreia Brandão,Manuela Pinheiro,Marta Cardoso,Adriana Resende,Joana Vieira,Carlos Palmeira,Gabriela Martins,Miguel Silva,Carla Pinto,Ana Peixoto,João Silva,Rui Henrique,Sofia Maia,Helder Maiato,Manuel R Teixeira,Paula Paulo

Biochimica et biophysica acta. Molecular cell research 1868:118929 PubMed33310066

2020

Tank Binding Kinase 1 modulates spindle assembly checkpoint components to regulate mitosis in breast and lung cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Meenu Maan,Neha Jaiswal Agrawal,Jaya Padmanabhan,Christelle Colin Leitzinger,Yainyrette Rivera-Rivera,Harold I Saavedra,Srikumar P Chellappan

Molecular cell 81:67-87.e9 PubMed33248027

2020

BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka Singh,Marion E Pesenti,Stefano Maffini,Sara Carmignani,Marius Hedtfeld,Arsen Petrovic,Anupallavi Srinivasamani,Tanja Bange,Andrea Musacchio

BMC cancer 17:861 PubMed29246203

2017

Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhe Zhang,Guojun Zhang,Zhipeng Gao,Shiguang Li,Zeliang Li,Jianbin Bi,Xiankui Liu,Zhenhua Li,Chuize Kong

Nature cell biology 17:1124-33 PubMed26258632

2015

Mad1 promotes chromosome congression by anchoring a kinesin motor to the kinetochore.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Akera,Yuhei Goto,Masamitsu Sato,Masayuki Yamamoto,Yoshinori Watanabe

The Journal of cell biology 209:221-34 PubMed25918224

2015

TRAMM/TrappC12 plays a role in chromosome congression, kinetochore stability, and CENP-E recruitment.

Applications

ICC/IF, WB

Species

Human, Human

Miroslav P Milev,Benedeta Hasaj,Djenann Saint-Dic,Sary Snounou,Qingchuan Zhao,Michael Sacher

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-BubR1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BubR1 antibody (AB70544)

Immunoprecipitation - Anti-BubR1 antibody (AB70544)

Western blot - Anti-BubR1 antibody (AB70544)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise