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AB237493

Anti-BUD31 antibody

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(1 Publication)

Rabbit Polyclonal BUD31 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BUD31.

View Alternative Names

EDG2, BUD31, Protein BUD31 homolog, Protein EDG-2, Protein G10 homolog

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (AB237493)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (AB237493)

Paraffin-embedded human spleen tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-BUD31 antibody (AB237493)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-BUD31 antibody (AB237493)

MCF7 (human breast adenocarcinoma cell line) cells stained for BUD31 (green) using ab237493 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (AB237493)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (AB237493)

Paraffin-embedded human colon cancer tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-BUD31 antibody (AB237493)
  • WB

Supplier Data

Western blot - Anti-BUD31 antibody (AB237493)

All lanes:

Western blot - Anti-BUD31 antibody (ab237493) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

Mouse liver lysate

Lane 3:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Lane 4:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

Lane 5:

K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate

Lane 6:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 7:

U-251 MG (human brain glioma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human BUD31.

P41223

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity greater than 95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BUD31 also known as Bud Site Selection Protein 31 is a component of the spliceosome machinery and plays an important role in RNA splicing. It weighs about 28 kilodaltons. This protein is expressed in various tissues including the brain and the heart. BUD31 is necessary for proper spliceosome assembly and facilitates mRNA processing by aiding in the removal of introns during pre-mRNA splicing.
Biological function summary

BUD31 interacts with multiple proteins as part of the spliceosomal complex particularly during the catalytic steps of splicing. This interaction is essential for maintaining the fidelity of the splicing process preventing errors that could result in defective or dysfunctional mRNA transcripts. Researchers have observed BUD31 involvement in cellular processes such as cell division and proliferation indicating its importance in cell cycle regulation.

Pathways

Multiple researchers have linked BUD31 to the RNA splicing pathway which is an important step in gene expression. BUD31 also interacts with other spliceosome-associated proteins like SF3B1 highlighting its role in spliceosome assembly and function. Another important pathway is the regulation of the cell cycle where BUD31's influence on mRNA processing affects the expression of genes essential for cell cycle progression. This interaction may influence how cells respond to signals that promote growth or initiate division.

Scientists have linked mutations or dysregulation of BUD31 to cancer. Research suggests that BUD31 impacts the splicing of mRNAs that encode for oncogenes and tumor suppressor genes potentially affecting tumorigenesis. Additionally changes in BUD31 expression could relate to neurodegenerative conditions due to its significant expression in the brain and role in proper mRNA processing. Connections with proteins like BRCA1 in the context of cancer suggest BUD31's influence on cell cycle checkpoints and DNA repair mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the pre-mRNA splicing process (PubMed : 28076346, PubMed : 28502770). May play a role as regulator of AR transcriptional activity; may increase AR transcriptional activity (PubMed : 25091737).
See full target information BUD31
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:10111 PubMed39578459

2024

Histone lactylation mediated by Fam172a in POMC neurons regulates energy balance.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuo Chen,Baocheng Wan,Hong Zhang,Lina Zhang,Rong Zhang,Lianxi Li,Yi Zhang,Cheng Hu
View all publications
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