Rabbit Polyclonal BUD31 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BUD31.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human BUD31. Database link P41223
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Zebrafish | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Zebrafish | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Cow, Zebrafish | Dilution info - | Notes - |
Target data
Function
Involved in the pre-mRNA splicing process (PubMed:28076346, PubMed:28502770). May play a role as regulator of AR transcriptional activity; may increase AR transcriptional activity (PubMed:25091737).
Alternative names
EDG2, BUD31, Protein BUD31 homolog, Protein EDG-2, Protein G10 homolog
Recommended products
Rabbit Polyclonal BUD31 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human BUD31.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human BUD31. Database link P41223
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity greater than 95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BUD31 also known as Bud Site Selection Protein 31 is a component of the spliceosome machinery and plays an important role in RNA splicing. It weighs about 28 kilodaltons. This protein is expressed in various tissues including the brain and the heart. BUD31 is necessary for proper spliceosome assembly and facilitates mRNA processing by aiding in the removal of introns during pre-mRNA splicing.
- Biological function summary
BUD31 interacts with multiple proteins as part of the spliceosomal complex particularly during the catalytic steps of splicing. This interaction is essential for maintaining the fidelity of the splicing process preventing errors that could result in defective or dysfunctional mRNA transcripts. Researchers have observed BUD31 involvement in cellular processes such as cell division and proliferation indicating its importance in cell cycle regulation.
- Pathways
Multiple researchers have linked BUD31 to the RNA splicing pathway which is an important step in gene expression. BUD31 also interacts with other spliceosome-associated proteins like SF3B1 highlighting its role in spliceosome assembly and function. Another important pathway is the regulation of the cell cycle where BUD31's influence on mRNA processing affects the expression of genes essential for cell cycle progression. This interaction may influence how cells respond to signals that promote growth or initiate division.
- Associated diseases and disorders
Scientists have linked mutations or dysregulation of BUD31 to cancer. Research suggests that BUD31 impacts the splicing of mRNAs that encode for oncogenes and tumor suppressor genes potentially affecting tumorigenesis. Additionally changes in BUD31 expression could relate to neurodegenerative conditions due to its significant expression in the brain and role in proper mRNA processing. Connections with proteins like BRCA1 in the context of cancer suggest BUD31's influence on cell cycle checkpoints and DNA repair mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-BUD31 antibody (ab237493)
All lanes: Western blot - Anti-BUD31 antibody (ab237493) at 1/500 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2: Mouse liver lysate
Lane 3: Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 4: NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lane 5: K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 6: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 7: U-251 MG (human brain glioma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (ab237493)
Paraffin-embedded human colon cancer tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BUD31 antibody (ab237493)
Paraffin-embedded human spleen tissue stained for BUD31 using ab237493 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-BUD31 antibody (ab237493)
MCF7 (human breast adenocarcinoma cell line) cells stained for BUD31 (green) using ab237493 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
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