AB17246

Anti-Butyrylcholinesterase antibody [3E8]

Name: Anti-Butyrylcholinesterase antibody [3E8] (ab17246) | Abcam
Brand: Abcam Limited
SKU: AB17246
Price: 525 USD
Availability: InStock

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(3 Publications)

Mouse Monoclonal Butyrylcholinesterase antibody. Suitable for ELISA and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Native Full Length Protein corresponding to Human BCHE.

View Alternative Names

CHE1, BCHE, Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II, Pseudocholinesterase

1 Images

ELISA - Anti-Butyrylcholinesterase antibody [3E8] (AB17246)

ELISA

Supplier Data

ELISA - Anti-Butyrylcholinesterase antibody [3E8] (AB17246)

Calibration curve of a sandwich assay for Butyrylcholinesterase using ab17246 as the capture antibody and a biotinylated detection antibody.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3E8

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ELISA

applications

Immunogen

Native Full Length Protein corresponding to Human BCHE.

P06276

Specificity

No reaction is seen with acetylcholinesterase from human nervous tissue and erythrocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1/8000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 2.9% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Store in the dark

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Butyrylcholinesterase often called BuChE or BChE is a serine hydrolase enzyme that catalyzes the hydrolysis of ester compounds such as butyrylcholine. It has a molecular mass of approximately 85 kDa. BChE is structurally similar to acetylcholinesterase (AChE) and is found in various tissues with high expression in the liver plasma and the central nervous system. This enzyme acts as a scavenger by breaking down circulating choline esters and certain therapeutic drugs playing an important role in detoxifying the blood.

Biological function summary

This enzyme is significant in nervous system function and is not restricted to acetylcholine breakdown but can hydrolyze larger ester molecules. It forms part of a complex regulatory mechanism alongside acetylcholinesterase ensuring precise modulating of neurotransmitter signaling. BChE can also serve as a backup to acetylcholinesterase in breaking down neurotransmitters therefore contributing to maintaining synaptic balance and functionality.

Pathways

BuChE is involved in the cholinergic signaling pathway playing a significant role in neurotransmitter regulation. This pathway is important for muscle activation and memory processing. BChE also impacts the metabolism of certain ester-type drugs used in anesthesia. Its activity balances with acetylcholinesterase modulating the availability of acetylcholine within synaptic clefts and seamlessly integrating into broader physiological processes.

Altered BChE activity correlates with Alzheimer's disease and certain liver diseases. In Alzheimer's decreased BChE activity parallels reduced acetylcholinesterase function which affects cholinergic transmission in the brain contributing to memory deficits. Disorders like liver cirrhosis also show a compromised BChE level due to liver's impaired enzyme production aligning it closely with liver function and disease diagnostics.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

See full target information BCHE

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 12:896 PubMed33563994

2021

Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Li,Barun Mahata,Mario Escobar,Jacob Goell,Kaiyuan Wang,Pranav Khemka,Isaac B Hilton

Alzheimer's research & therapy 11:80 PubMed31511061

2019

Visual hallucinations in Alzheimer's disease do not seem to be associated with chronic hypoperfusion of to visual processing areas V2 and V3 but may be associated with reduced cholinergic input to these areas.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsey Isla Sinclair,Amit Kumar,Taher Darreh-Shori,Seth Love

Biosensors & bioelectronics 104:39-44 PubMed29306031

2018

Simultaneous detection of dual biomarkers from humans exposed to organophosphorus pesticides by combination of immunochromatographic test strip and ellman assay.

Applications

Unspecified application

Species

Unspecified reactive species

Mingming Yang,Yuting Zhao,Limin Wang,Michael Paulsen,Christopher D Simpson,Fengquan Liu,Dan Du,Yuehe Lin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Butyrylcholinesterase antibody [3E8]

ELISA - Anti-Butyrylcholinesterase antibody [3E8] (AB17246)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise