Skip to main content

Rabbit Recombinant Monoclonal Butyrylcholinesterase antibody. Suitable for WB and reacts with Mouse, Rat samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (AB274375), expandable thumbnail
  • Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (AB274375), expandable thumbnail
  • Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (AB274375), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PIHC-Fr
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Tested
Not recommended
Not recommended

Not recommended
Not recommended

Species
Rat, Mouse, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Target data

Function

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

Alternative names

Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II, Pseudocholinesterase, Bche

Recommended products

Rabbit Recombinant Monoclonal Butyrylcholinesterase antibody. Suitable for WB and reacts with Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23792-163
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Butyrylcholinesterase often called BuChE or BChE is a serine hydrolase enzyme that catalyzes the hydrolysis of ester compounds such as butyrylcholine. It has a molecular mass of approximately 85 kDa. BChE is structurally similar to acetylcholinesterase (AChE) and is found in various tissues with high expression in the liver plasma and the central nervous system. This enzyme acts as a scavenger by breaking down circulating choline esters and certain therapeutic drugs playing an important role in detoxifying the blood.

Biological function summary

This enzyme is significant in nervous system function and is not restricted to acetylcholine breakdown but can hydrolyze larger ester molecules. It forms part of a complex regulatory mechanism alongside acetylcholinesterase ensuring precise modulating of neurotransmitter signaling. BChE can also serve as a backup to acetylcholinesterase in breaking down neurotransmitters therefore contributing to maintaining synaptic balance and functionality.

Pathways

BuChE is involved in the cholinergic signaling pathway playing a significant role in neurotransmitter regulation. This pathway is important for muscle activation and memory processing. BChE also impacts the metabolism of certain ester-type drugs used in anesthesia. Its activity balances with acetylcholinesterase modulating the availability of acetylcholine within synaptic clefts and seamlessly integrating into broader physiological processes.

Associated diseases and disorders

Altered BChE activity correlates with Alzheimer's disease and certain liver diseases. In Alzheimer's decreased BChE activity parallels reduced acetylcholinesterase function which affects cholinergic transmission in the brain contributing to memory deficits. Disorders like liver cirrhosis also show a compromised BChE level due to liver's impaired enzyme production aligning it closely with liver function and disease diagnostics.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375), expandable thumbnail

    Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Butyrylcholinesterase is a glycoprotein. The molecular weight observed is consistent with what have been described in literature (PMID: 16434405, PMID: 24618259).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure times: Lane 1-3: 89 seconds Lane 4: 3 minutes.

    All lanes: Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse lung tissue lysate at 20 µg

    Lane 4: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa

    Observed band size: 76 kDa

  • Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375), expandable thumbnail

    Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Butyrylcholinesterase is a glycoprotein. The molecular weight observed is consistent with what have been described in literature (PMID: 16434405, PMID: 24618259).

    Negative control: PC-12 (PMID: 7842168).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375) at 1/1000 dilution

    Lane 1: Rat liver tissue lysate at 20 µg

    Lane 2: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa

    Observed band size: 76 kDa

  • Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375), expandable thumbnail

    Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Butyrylcholinesterase is a glycoprotein. The molecular weight observed is consistent with what have been described in literature (PMID: 16434405, PMID: 24618259).

    Negative control: NIH/3T3 (PMID: 7842168).

    Exposure times: 3 minutes.

    All lanes: Western blot - Anti-Butyrylcholinesterase antibody [EPR23792-163] (ab274375) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate at 20 µg

    Lane 2: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 68 kDa

    Observed band size: 76 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com