AB192925

Anti-BVR antibody

Name: Anti-BVR antibody (ab192925) | Abcam
Brand: Abcam Limited
SKU: AB192925

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(3 Publications)

Rabbit Polyclonal BVR antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human BLVRA aa 50-150.

View Alternative Names

BLVR, BVR, BLVRA, Biliverdin reductase A, BVR A, Biliverdin-IX alpha-reductase

2 Images

Supplier Data

Immunoprecipitation - Anti-BVR antibody (AB192925)

Detection of BVR in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab192925 at 6 μg/reaction for IP and 1μg/ml for WB detection.

Detection : Chemiluminescence with an exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-BVR antibody (ab192925)

Predicted band size: 33 kDa

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Supplier Data

Western blot - Anti-BVR antibody (AB192925)

All lanes:

Western blot - Anti-BVR antibody (ab192925) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 33 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human BLVRA aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P53004

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192925 was affinity purified using an epitope specific to BVR immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The BVR protein known in full form as Biliverdin Reductase plays an essential mechanical role in the conversion of biliverdin to bilirubin. It exists as two main isoforms BVR-A and BVR-B with molecular masses approximately of 33 kDa. BVR is widely expressed in various tissues with higher levels found in liver kidney and spleen. As an enzyme its main function centers around the redox cycle between biliverdin and bilirubin providing an antioxidant defense mechanism.

Biological function summary

BVR influences cell signaling and growth processes acting as a multifunctional enzyme beyond its primary reductive activity. It participates in the MAPK and PI3K/Akt signaling pathways acting both as a kinase and phosphatase which affects transcription factor regulation. BVR is also involved in heme metabolism working in conjunction with heme oxygenase to recycle heme a vital cellular molecule.

Pathways

BVR significantly contributes to the antioxidative and anti-inflammatory pathways. It operates within the heme catabolic pathway and the MAPK signaling cascade linking to proteins like heme oxygenase-1 (HO-1) and the transcription factor Nrf2. These pathways support cell response to oxidative stress and regulate gene expression in response to environmental stressors.

BVR is associated with conditions such as jaundice and chronic inflammation-oriented diseases. Its interaction with proteins like heme oxygenase-1 (HO-1) elevates its importance in managing oxidative stress-related disorders. Disruption in BVR function or expression can influence bilirubin metabolism potentially leading to hyperbilirubinemia and contributing to inflammatory disease development through altered cell signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor (PubMed : 10858451, PubMed : 7929092, PubMed : 8424666, PubMed : 8631357). Does not reduce bilirubin IXbeta (PubMed : 10858451). Uses the reactants NADH or NADPH depending on the pH; NADH is used at the acidic pH range (6-6.9) and NADPH at the alkaline range (8.5-8.7) (PubMed : 7929092, PubMed : 8424666, PubMed : 8631357). NADPH, however, is the probable reactant in biological systems (PubMed : 7929092).

See full target information BLVRA

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

mBio 16:e0333124 PubMed39611845

2024

Coatomer complex I is required for the transport of SARS-CoV-2 progeny virions from the endoplasmic reticulum-Golgi intermediate compartment.

Applications

Unspecified application

Species

Unspecified reactive species

Ai Hirabayashi,Yukiko Muramoto,Toru Takenaga,Yugo Tsunoda,Mayumi Wakazaki,Mayuko Sato,Yoko Fujita-Fujiharu,Norimichi Nomura,Koji Yamauchi,Chiho Onishi,Masahiro Nakano,Kiminori Toyooka,Takeshi Noda

Biomolecules & therapeutics 31:674-681 PubMed37558633

2023

Diagnostic Role of Bile Pigment Components in Biliary Tract Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Keun Soo Ahn,Koo Jeong Kang,Yong Hoon Kim,Tae-Seok Kim,Kwang Bum Cho,Hye Soon Kim,Won-Ki Baek,Seong-Il Suh,Jin-Yi Han

American journal of cancer research 11:4844-4865 PubMed34765296

2021

LncRNA NR2F2-AS1 induces epithelial-mesenchymal transition of non-small cell lung cancer by modulating BVR/ATF-2 pathway via regulating miR-545-5p/c-Met axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Liu,Qun-Gen Li,Yang Zhou,Ying-Yue Cao,Zi-Xin Wei,Ying-Hua Jin,Xin Wang,Ying-Ying Chen,Li Qi,Jian-Xiong Geng,Fang Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-BVR antibody

Immunoprecipitation - Anti-BVR antibody (AB192925)

Western blot - Anti-BVR antibody (AB192925)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise