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AB194961

Anti-BYSL antibody

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(2 Publications)

Rabbit Polyclonal BYSL antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BYSL aa 1-50.

View Alternative Names

ENP1, BYSL, Bystin

1 Images
Western blot - Anti-BYSL antibody (AB194961)
  • WB

Supplier Data

Western blot - Anti-BYSL antibody (AB194961)

Cell lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-BYSL antibody (ab194961) at 0.4 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Predicted band size: 50 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human BYSL aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q13895

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target BYSL also known as Bystin plays an important role in cell division and growth by participating in ribosomal RNA (rRNA) processing. BYSL has a molecular mass of about 58 kDa. Expression of BYSL occurs mainly in dividing tissues such as the testis and tumor cells where cell proliferation is high. The protein's function involves interaction with other ribonucleoproteins to facilitate the maturation of pre-ribosomal particles.
Biological function summary

BYSL is essential for the assembly of the 90S preribosome a precursor complex pivotal to eukaryotic ribosome biogenesis. It acts alongside other nucleolar proteins to support the assembly and processing of the 18S rRNA necessary for the small ribosomal subunit. Without proper function of BYSL cells experience defects in ribosomal assembly affecting overall protein synthesis.

Pathways

BYSL functions integrally in the ribosome biogenesis pathway a central component of cellular growth and proliferation. It works closely with proteins like NOP58 and UTP18 forming part of the U3 ribonucleoprotein complex essential for early pre-rRNA processing. Another significant pathway is the nucleolar processing of Pre-47S rRNA where BYSL directs ribosome maturation processes ensuring efficient synthesis and cell growth.

Abnormalities in BYSL function associate with various cancers emphasizing its connections to uncontrolled cell proliferation. Overexpression or dysfunction of BYSL correlates with tumorigenic processes linking it to proteins such as C-MYC and p53 which also play roles in cancer development. BYSL also relates to the disorder of ribosomopathy where impaired ribosome biogenesis leads to pathological conditions suggesting disruptions that affect overall cellular homeostasis and growth dynamics.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits. May be required for trophinin-dependent regulation of cell adhesion during implantation of human embryos.
See full target information BYSL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:33-43 PubMed36300343

2022

AKBA inhibits radiotherapy resistance in lung cancer by inhibiting maspin methylation and regulating the AKT/FOXO1/p21 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Gong,Wei Li,Jing Wu,Yao-Yao Li,Yi Ma,Li-Wen Tang

Cell death and differentiation 29:687-696 PubMed34611297

2021

RNA-interference screen for p53 regulators unveils a role of WDR75 in ribosome biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Pavel Moudry,Katarina Chroma,Sladana Bursac,Sinisa Volarevic,Jiri Bartek
View all publications
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