AB85090

Anti-BZW1/Protein Orf antibody

Name: Anti-BZW1/Protein Orf antibody (ab85090) | Abcam
Brand: Abcam Limited
SKU: AB85090
Price: 525 USD
Availability: InStock
Rating: 4 (4 reviews)

(4 Reviews)

(6 Publications)

Rabbit Polyclonal BZW1/Protein Orf antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human BZW1.

View Alternative Names

5MP2, BZAP45, KIAA0005, BZW1, eIF5-mimic protein 2, Basic leucine zipper and W2 domain-containing protein 1, Protein Orf

2 Images

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Western blot - Anti-BZW1/Protein Orf antibody (AB85090)

All lanes:

Western blot - Anti-BZW1/Protein Orf antibody (ab85090) at 1/500 dilution

Lane 1:

HepG2 cell lysate

Lane 2:

HeLa cell lysate

Lane 3:

MCF7 cell lysate

Predicted band size: 48 kDa

Observed band size: 48 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BZW1/Protein Orf antibody (AB85090)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BZW1/Protein Orf antibody (AB85090)

Immunohistochemical analysis of BZW1/Protein Orf expression in paraffin embedded formalin fixed lymph node section using 1/100 ab85090. note cytoplasmic staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human BZW1. The exact immunogen used to generate this antibody is proprietary information.

Q7L1Q6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/250 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Lyophilized

Reconstitution

reconstitute with water at 200µL

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BZW1 also known as Protein Orf is a member of the basic leucine zipper (bZIP) family of proteins. The mass of BZW1 is approximately 42 kDa. It is mainly expressed in the nucleus of cells. BZW1 plays a role in the regulation of transcription and translation processes. This regulation occurs through its interaction with specific sequences on the DNA and RNA contributing to the modification of gene expression.

Biological function summary

BZW1 influences cell growth and proliferation by engaging in the regulation of gene transcription. It can interact with other proteins to form complexes that modulate these processes. BZW1 adjusts protein synthesis rates by acting at the level of translational control affecting cellular responses to different growth conditions. Its function supports overall cellular homeostasis by ensuring proteins are produced as needed.

Pathways

BZW1 is integral to both the mTOR and eIF2 signaling pathways which are critical in regulating protein synthesis and stress responses. It is related to other proteins like mTOR and eIF2α within these pathways and its activity can influence how cells respond to nutrient availability and environmental changes.

Alterations in BZW1 expression have associations with cancer and neurodegenerative diseases. In cancer dysregulation of BZW1 can affect the cell cycle by impacting the proteins involved like p53 thereby promoting uncontrolled cell proliferation. In neurodegenerative diseases changes in its function can disrupt protein homeostasis leading to the accumulation of proteins that contribute to disease progression and pathways involving proteins like α-synuclein.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Translation initiation regulator which represses repeat-associated non-AUG (RAN) initiated translation probably by acting as a competitive inhibitor of eukaryotic translation initiation factor 5 (EIF5) function (PubMed : 29470543, PubMed : 34260931). Enhances histone H4 gene transcription but does not seem to bind DNA directly (PubMed : 11524015).

See full target information BZW1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in genetics 13:1002673 PubMed36267402

2022

Expression, oncological and immunological characterizations of BZW1/2 in pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiachen Ge,Senmao Mu,Erwei Xiao,Guangjin Tian,Lianyuan Tao,Deyu Li

Journal of gastroenterology and hepatology 37:144-153 PubMed34370878

2021

Long non-coding RNA TMPO-AS1 facilitates the progression of colorectal cancer cells via sponging miR-98-5p to upregulate BCAT1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jinjun Ye,Yukuang Yan,Le Xin,Jidong Liu,Tao Tang,Xing Bao

Molecular medicine reports 22:3347-3357 PubMed32945505

2020

Long non‑coding RNA NEAT1 modifies cell proliferation, colony formation, apoptosis, migration and invasion via the miR‑4500/BZW1 axis in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hongying Xu,Xiaowei Sun,Ying Huang,Qin Si,Maokun Li

Scientific reports 9:14624 PubMed31601833

2019

Overexpression of BZW1 is an independent poor prognosis marker and its down-regulation suppresses lung adenocarcinoma metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jean Chiou,Yu-Chan Chang,Yi-Hua Jan,Hsing-Fang Tsai,Chih-Jen Yang,Ming-Shyan Huang,Yung-Luen Yu,Michael Hsiao

International journal of clinical and experimental 11:5901-5908 PubMed31949677

2018

MicroRNA-129-3p functions as a tumor suppressor in serous ovarian cancer by targeting BZW1.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Liu,Hongxi Zhao,Li Gong,Li Yao,Yanhong Li,Wei Zhang

PloS one 13:e0192648 PubMed29470543

2018

Translational autoregulation of BZW1 and BZW2 expression by modulating the stringency of start codon selection.

Applications

Unspecified application

Species

Unspecified reactive species

Gary Loughran,Andrew E Firth,John F Atkins,Ivaylo P Ivanov

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-BZW1/Protein Orf antibody

Western blot - Anti-BZW1/Protein Orf antibody (AB85090)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BZW1/Protein Orf antibody (AB85090)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Reconstitution

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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