AB254772

Anti-BZW2 antibody

Name: Anti-BZW2 antibody (ab254772) | Abcam
Brand: Abcam Limited
SKU: AB254772

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(4 Publications)

Rabbit Polyclonal BZW2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BZW2 aa 150-250.

View Alternative Names

5MP1, HSPC028, MSTP017, BZW2, eIF5-mimic protein 1, Basic leucine zipper and W2 domain-containing protein 2

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-BZW2 antibody (AB254772)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BZW2 antibody (AB254772)

PFA fixed, Triton X-100 permeabilized U-2 OS (Human bone osteosarcoma epithelial cell line) cells labeling BZW2 using ab254772 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BZW2 antibody (AB254772)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BZW2 antibody (AB254772)

Paraffin-embedded human pancreas tissue stained for BZW2 using ab254772 at 1/20 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-BZW2 antibody (AB254772)

Western blot analysis in human cell line SK-BR-3.

All lanes:

Western blot - Anti-BZW2 antibody (ab254772)

All lanes:

SK-BR-3 (Human mammary gland adenocarcinoma cell line) whole cell lysate

Predicted band size: 48 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human BZW2 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6E2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BZW2 also known as Basic Leucine Zipper and W2 Domain-Containing Protein 2 is a leucine zipper protein involved in the regulation of translation. It has other names like HSPC017 and 25FL. The molecular weight of BZW2 is approximately 46 kDa. This protein is expressed in various tissues with high levels in the pancreas and placenta. Its key mechanical role involves interacting with ribosomal subunits to modulate translation initiation particularly under conditions that require altered protein synthesis rates.

Biological function summary

Several critical roles situate BZW2 in cellular growth and proliferation. It acts as a translation regulator and integrates into ribosomal complexes during the protein synthesis process. BZW2 augments the synthesis of proteins by accelerating the assembly of initiation complexes. Furthermore BZW2 indirectly affects cell cycle progression by coordinating with other translational regulators. Its expression levels correlate with rapidly dividing tissues hinting at its role in growth and development.

Pathways

BZW2 plays an integral part in the mTOR signaling pathway and the integrated stress response (ISR) pathway. In the mTOR pathway BZW2 collaborates with other initiation factors to enhance protein synthesis related to cellular growth. Within the ISR it adjusts protein translation in response to cellular stress maintaining balance in protein homeostasis. BZW2's interaction with proteins like eIF2α highlights its significance in translational control during stress conditions.

Alterations in BZW2 expression relate to cancer and neurodegenerative disorders. Abnormal regulation of BZW2 can lead to increased cell proliferation linking it to cancer development particularly in aggressive tumor types. It also connects with proteins like p53 in cancer pathways affecting apoptosis. Additionally dysfunction in the ISR involving BZW2 has implications in neurodegenerative diseases potentially through its role in stress response and protein folding affecting neuronal survival.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Translation initiation regulator which represses non-AUG initiated translation and repeat-associated non-AUG (RAN) initiated translation by acting as a competitive inhibitor of eukaryotic translation initiation factor 5 (EIF5) function (PubMed : 21745818, PubMed : 28981728, PubMed : 29470543, PubMed : 34260931). Increases the accuracy of translation initiation by impeding EIF5-dependent translation from non-AUG codons by competing with it for interaction with EIF2S2 within the 43S pre-initiation complex (PIC) in an EIF3C-binding dependent manner (PubMed : 21745818, PubMed : 28981728, PubMed : 34260931).

See full target information BZW2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Disease markers 2023:5899662 PubMed36644609

2023

TRIM58 Interacts with ZEB1 to Suppress NSCLC Tumor Malignancy by Promoting ZEB1 Protein Degradation via UPP.

Applications

Unspecified application

Species

Unspecified reactive species

Rongxin Shang,Jiakuan Chen,Yang Gao,Jijun Chen,Guoliang Han

Clinics (Sao Paulo, Brazil) 78:100129 PubMed36473368

2022

Mesenchymal stem cells-derived exosomal miR-653-5p suppresses laryngeal papilloma progression by inhibiting BZW2.

Applications

Unspecified application

Species

Unspecified reactive species

Binya Hu,Min Huang,Lihua Tao,Yun Li,Yuting Kuang,Guangliang Liu,Sijun Zhao

Frontiers in genetics 13:1002673 PubMed36267402

2022

Expression, oncological and immunological characterizations of BZW1/2 in pancreatic adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jiachen Ge,Senmao Mu,Erwei Xiao,Guangjin Tian,Lianyuan Tao,Deyu Li

Journal of Cancer 12:5125-5135 PubMed34335929

2021

BZW2/5MP1 acts as a promising target in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Guoxiong Li,Anqian Lu,Anna Chen,Shuang Geng,Yu Xu,Xin Chen,Jin Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-BZW2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-BZW2 antibody (AB254772)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BZW2 antibody (AB254772)

Western blot - Anti-BZW2 antibody (AB254772)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise