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AB96682

Anti-BZW2 antibody

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(2 Publications)

Rabbit Polyclonal BZW2 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human BZW2 aa 1-250.

View Alternative Names

5MP1, HSPC028, MSTP017, BZW2, eIF5-mimic protein 1, Basic leucine zipper and W2 domain-containing protein 2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-BZW2 antibody (AB96682)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BZW2 antibody (AB96682)

ab96682, at 1/200 dilution, staining BZW2 in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.

Western blot - Anti-BZW2 antibody (AB96682)
  • WB

Unknown

Western blot - Anti-BZW2 antibody (AB96682)

10% SDS-PAGE

All lanes:

Western blot - Anti-BZW2 antibody (ab96682) at 1/1000 dilution

Lane 1:

A431 whole cell lysate at 30 µg

Lane 2:

HeLa S3 whole cell lysate at 30 µg

Lane 3:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 48 kDa

false

Western blot - Anti-BZW2 antibody (AB96682)
  • WB

Supplier Data

Western blot - Anti-BZW2 antibody (AB96682)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-BZW2 antibody (ab96682) at 1/1000 dilution

Lane 1:

Neuro2A whole cell lysates at 30 µg

Lane 2:

C8D30 whole cell lysates at 30 µg

Predicted band size: 48 kDa

false

Western blot - Anti-BZW2 antibody (AB96682)
  • WB

Supplier Data

Western blot - Anti-BZW2 antibody (AB96682)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-BZW2 antibody (ab96682) at 1/1000 dilution

All lanes:

Rat2 whole cell lysate at 30 µg

Predicted band size: 48 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human BZW2 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6E2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BZW2 also known as Basic Leucine Zipper and W2 Domain-Containing Protein 2 is a leucine zipper protein involved in the regulation of translation. It has other names like HSPC017 and 25FL. The molecular weight of BZW2 is approximately 46 kDa. This protein is expressed in various tissues with high levels in the pancreas and placenta. Its key mechanical role involves interacting with ribosomal subunits to modulate translation initiation particularly under conditions that require altered protein synthesis rates.
Biological function summary

Several critical roles situate BZW2 in cellular growth and proliferation. It acts as a translation regulator and integrates into ribosomal complexes during the protein synthesis process. BZW2 augments the synthesis of proteins by accelerating the assembly of initiation complexes. Furthermore BZW2 indirectly affects cell cycle progression by coordinating with other translational regulators. Its expression levels correlate with rapidly dividing tissues hinting at its role in growth and development.

Pathways

BZW2 plays an integral part in the mTOR signaling pathway and the integrated stress response (ISR) pathway. In the mTOR pathway BZW2 collaborates with other initiation factors to enhance protein synthesis related to cellular growth. Within the ISR it adjusts protein translation in response to cellular stress maintaining balance in protein homeostasis. BZW2's interaction with proteins like eIF2α highlights its significance in translational control during stress conditions.

Alterations in BZW2 expression relate to cancer and neurodegenerative disorders. Abnormal regulation of BZW2 can lead to increased cell proliferation linking it to cancer development particularly in aggressive tumor types. It also connects with proteins like p53 in cancer pathways affecting apoptosis. Additionally dysfunction in the ISR involving BZW2 has implications in neurodegenerative diseases potentially through its role in stress response and protein folding affecting neuronal survival.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Translation initiation regulator which represses non-AUG initiated translation and repeat-associated non-AUG (RAN) initiated translation by acting as a competitive inhibitor of eukaryotic translation initiation factor 5 (EIF5) function (PubMed : 21745818, PubMed : 28981728, PubMed : 29470543, PubMed : 34260931). Increases the accuracy of translation initiation by impeding EIF5-dependent translation from non-AUG codons by competing with it for interaction with EIF2S2 within the 43S pre-initiation complex (PIC) in an EIF3C-binding dependent manner (PubMed : 21745818, PubMed : 28981728, PubMed : 34260931).
See full target information BZW2
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular & cellular proteomics : MCP 23:100709 PubMed38154691

2023

Interactome Analysis Identifies the Role of BZW2 in Promoting Endoplasmic Reticulum-Mitochondria Contact and Mitochondrial Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

George Maio,Mike Smith,Ruchika Bhawal,Sheng Zhang,Jeremy M Baskin,Jenny Li,Hening Lin

PloS one 13:e0192648 PubMed29470543

2018

Translational autoregulation of BZW1 and BZW2 expression by modulating the stringency of start codon selection.

Applications

Unspecified application

Species

Unspecified reactive species

Gary Loughran,Andrew E Firth,John F Atkins,Ivaylo P Ivanov
View all publications
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