Anti-c-Fos antibody [EPR20769] ab214672 is a rabbit monoclonal antibody that is used in c-Fos western blotting and immunofluorescence. Suitable for human and mouse samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR20769 is cited in over 20 publications
Images
![Immunoprecipitation - Anti-c-Fos antibody [EPR20769] (AB214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--immunoprecipitation-img303.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-c-Fos antibody [EPR20769] (AB214672)")
![Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] (AB214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--immunocytochemistry-immunofluorescence-img45757.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] (AB214672)")
![Western blot - Anti-c-Fos antibody [EPR20769] (AB214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--western-blot-img148230.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-c-Fos antibody [EPR20769] (AB214672)")
![Western blot - Anti-c-Fos antibody [EPR20769] (AB214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--western-blot-img159053.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-c-Fos antibody [EPR20769] (AB214672)")
![Western blot - Anti-c-Fos antibody [EPR20769] (AB214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--western-blot-img146185.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-c-Fos antibody [EPR20769] (AB214672)")
Publications
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- Frontiers in nutrition 10:12789062023Energy deficiency promotes rhythmic foraging behavior by activating neurons in paraventricular hypothalamic nucleus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanshan Wu,Jing Wang,Yang Xu,Zicheng Zhang,Xinchen Jin,Yixiao Liang,Yueping Ge,Huidong Zhan,Li Peng,Dandan Luo,Mengzhu Li,Wenkai Bi,Qingbo Guan,Zhao HePubMed 37899828
- Translational psychiatry 13:172023Fecal microbiota transplantation improves VPA-induced ASD mice by modulating the serotonergic and glutamatergic synapse signaling pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJifeng Wang,Yuan Cao,Weiliang Hou,Dexi Bi,Fang Yin,Yaohui Gao,Dengfeng Huang,Yingying Li,Zhan Cao,Yinmei Yan,Jianhua Zhao,Dewu Kong,Xiaoqiong Lv,Linsheng Huang,Hui Zhong,Chunyan Wu,Qiyi Chen,Rong Yang,Qing Wei,Huanlong QinPubMed 36670104
- Frontiers in neuroscience 16:10195902022Moxibustion alleviates depression-like behavior in rats with Crohn's disease by inhibiting the kynurenine pathway metabolism in the gut-brain axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChunhui Bao,Jin Huang,Huangan Wu,Yueying Ma,Hongyu Zhou,Liming Chen,Dandan Yang,Huirong Liu,Yin Shi,Yuan LuPubMed 36570839
- Neuroscience bulletin 39:617-6302022Basal Forebrain Cholinergic Innervation Induces Depression-Like Behaviors Through Ventral Subiculum Hyperactivation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNana Yu,Huina Song,Guangpin Chu,Xu Zhan,Bo Liu,Yangling Mu,Jian-Zhi Wang,Yisheng LuPubMed 36342657
- Biomedicines 10:2022Isoflurane Rescue Schizophrenia-Related Deficits through Parvalbumin-Positive Neurons in the Dentate Gyrus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHualing Peng,Jie Jia,Yisheng Lu,Hua ZhengPubMed 36359279
- Frontiers in endocrinology 13:9327612022Involvement of POMC neurons in LEAP2 regulation of food intake and body weight.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangpin Chu,Hualing Peng,Nana Yu,Yuejin Zhang,Xueling Lin,Yisheng LuPubMed 36387867
- Nature 610:319-3262022Maturation and circuit integration of transplanted human cortical organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOmer Revah,Felicity Gore,Kevin W Kelley,Jimena Andersen,Noriaki Sakai,Xiaoyu Chen,Min-Yin Li,Fikri Birey,Xiao Yang,Nay L Saw,Samuel W Baker,Neal D Amin,Shravanti Kulkarni,Rachana Mudipalli,Bianxiao Cui,Seiji Nishino,Gerald A Grant,Juliet K Knowles,Mehrdad Shamloo,John R Huguenard,Karl Deisseroth,Sergiu P PașcaPubMed 36224417
- Pharmacological research 182:1063322022Lactobacillus plantarum-derived extracellular vesicles protect against ischemic brain injury via the microRNA-101a-3p/c-Fos/TGF-β axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhang Yang,Zidan Gao,Zhennai Yang,Yifan Zhang,Hongqun Chen,Xuexia Yang,Xuming Fang,Yingwu Zhu,Jiayan Zhang,Fu Ouyang,Jun Li,Gang Cai,Yuan Li,Xiang Lin,Ruihan Ni,Chong Xia,Ruihua Wang,Xiaofang Shi,Lan ChuPubMed 35779817
- Nature communications 13:17992022Brain-wide mapping reveals that engrams for a single memory are distributed across multiple brain regions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDheeraj S Roy,Young-Gyun Park,Minyoung E Kim,Ying Zhang,Sachie K Ogawa,Nicholas DiNapoli,Xinyi Gu,Jae H Cho,Heejin Choi,Lee Kamentsky,Jared Martin,Olivia Mosto,Tomomi Aida,Kwanghun Chung,Susumu TonegawaPubMed 35379803
- Frontiers in cell and developmental biology 10:7705502022An Aging-Related Gene Signature-Based Model for Risk Stratification and Prognosis Prediction in Lung Squamous Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWen-Yu Zhai,Fang-Fang Duan,Si Chen,Jun-Ye Wang,Ze-Rui Zhao,Yi-Zhi Wang,Bing-Yu Rao,Yao-Bin Lin,Hao LongPubMed 35300428
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected |
| Common marmoset | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Common marmoset | Dilution info - | Notes - |
Associated Products
Select an associated product type
11 products for Alternative Product
Target data
Function
Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
Alternative names
Proto-oncogene c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, G0S7, FOS
Recommended products
Anti-c-Fos antibody [EPR20769] ab214672 is a rabbit monoclonal antibody that is used in c-Fos western blotting and immunofluorescence. Suitable for human and mouse samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EPR20769 is cited in over 20 publications
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR20769
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.
- Biological function summary
C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.
- Pathways
C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.
- Associated diseases and disorders
Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-c-Fos antibody [EPR20769] (ab214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--immunoprecipitation-img303.jpg "Immunoprecipitation - Anti-c-Fos antibody [EPR20769] (ab214672)")
Immunoprecipitation - Anti-c-Fos antibody [EPR20769] (ab214672)
c-Fos was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab214672 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab214672 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) grown in serum free medium for 36 hours, followed by addition of 20%FBS for 2 hours, whole cell lysate, 10 μg (Input).
Lane 2: ab214672 IP in HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab214672 in HeLa grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours, whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.The observed lower band is a proteasomal degradation fragment (PMID: 9737957).
All lanes: Immunoprecipitation - Anti-c-Fos antibody [EPR20769] (ab214672)
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 55-60 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] (ab214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--immunocytochemistry-immunofluorescence-img45757.jpg "Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] (ab214672)")
Immunocytochemistry/ Immunofluorescence - Anti-c-Fos antibody [EPR20769] (ab214672)
Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized serum treated and non-treated HeLa (human cervix adenocarcinoma epithelial cell) cells labeling c-Fos with ab214672 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing weakly nuclear staining on HeLa cells grown in serum free medium for 36 hours. Expression of c-Fos increased in HeLa cells grown in serum free medium for 36 hours followed by addition of 20% FBS for 2 hours.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
![Western blot - Anti-c-Fos antibody [EPR20769] (ab214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--western-blot-img148230.jpg "Western blot - Anti-c-Fos antibody [EPR20769] (ab214672)")
Western blot - Anti-c-Fos antibody [EPR20769] (ab214672)
Exposure time: 15 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression of c-Fos is induced by the addition of 20%FBS (PMID: 24386331, PMID: 23300800, PMID: 25695333).
All lanes: Western blot - Anti-c-Fos antibody [EPR20769] (ab214672) at 1/1000 dilution
Lane 1: Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) grown in serum free medium for 36 hours, whole cell lysate at 10 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) grown in serum free medium for 36 hours, followed by addition of 20% FBS for 2 hours, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 55-60 kDa
![Western blot - Anti-c-Fos antibody [EPR20769] (ab214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--western-blot-img159053.jpg "Western blot - Anti-c-Fos antibody [EPR20769] (ab214672)")
Western blot - Anti-c-Fos antibody [EPR20769] (ab214672)
Exposure time: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
PMA treatment induces expression of c-Fos, as documented in the literature (PMID: 24386331, PMID: 23300800, PMID: 25695333).
All lanes: Western blot - Anti-c-Fos antibody [EPR20769] (ab214672) at 1/5000 dilution
Lane 1: Untreated Jurkat (human T cell leukemia cell line from peripheral blood) grown in serum free medium overnight, whole cell lysate at 10 µg
Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) grown in serum free medium overnight, followed by treatment with 200 nM PMA for 4 hours, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 55-60 kDa
![Western blot - Anti-c-Fos antibody [EPR20769] (ab214672), expandable thumbnail](https://content.abcam.com/products/images/c-fos-antibody-epr20769-ab214672--western-blot-img146185.jpg "Western blot - Anti-c-Fos antibody [EPR20769] (ab214672)")
Western blot - Anti-c-Fos antibody [EPR20769] (ab214672)
Exposure time: 1 minute.
Blocking/Dilution buffer: 5% NFDM/TBST.
PMA treatment induces expression of c-Fos, as documented in the literature (PMID: 24386331, PMID: 23300800, PMID: 25695333).
All lanes: Western blot - Anti-c-Fos antibody [EPR20769] (ab214672) at 1/5000 dilution
Lane 1: Untreated RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus grown in serum free medium overnight, whole cell lysate at 10 µg
Lane 2: RAW264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) grown in serum free medium overnight, followed by treatment with 200 nM PMA for 4 hours, whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 55-60 kDa
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