AB267451

Anti-c-Fos antibody [EPR20769] - BSA and Azide free (Capture)

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal c-Fos antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.

View Alternative Names

G0S7, FOS, Protein c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos

1 Images

Sandwich ELISA - Anti-c-Fos antibody [EPR20769] - BSA and Azide free (Capture) (AB267451)

sELISA

Supplier Data

Sandwich ELISA - Anti-c-Fos antibody [EPR20769] - BSA and Azide free (Capture) (AB267451)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab264626).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20769

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

style

left-column

Product details

ab267451 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human c-Fos. The recommended pair for sandwich ELISA is:
Capture: ab267451, Human c-Fos Capture Antibody (unconjugated)
Detector: ab267452, Human c-Fos Detector Antibody (unconjugated)
The reference range value is 54.6875 - 3500 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.

Biological function summary

C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.

Pathways

C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.

Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.

See full target information FOS

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-c-Fos antibody [EPR20769] - BSA and Azide free (Capture)

Sandwich ELISA - Anti-c-Fos antibody [EPR20769] - BSA and Azide free (Capture) (AB267451)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise