Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (AB289726)

This data was generated with ab289723, the same antibody in a different formulation.

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labelling c-Fos at 1/100 (5.46 μg/ml) for 30 mins at room temperature, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining on rat hippocampus. The nuclear counterstain is Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (AB289726)

This data was generated with ab289723, the same antibody in a different formulation.

Immunohistochemical analysis of paraffin-embedded rat hypothalamus tissue labelling c-Fos at 1/100 (5.46μg/ml) for 30 mins at room temperature, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining on rat hypothalamus. The nuclear counterstain is Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (AB289726)

This data was generated with ab289723, the same antibody in a different formulation.

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labelling c-Fos at 1/100 (5.46μg/ml) for 30 mins at room temperature, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection). Nuclear staining on rat cerebrum. The nuclear counterstain is Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, followed by ready to use LeicaDS9800 (BOND™ Polymer Refine Detection).

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IP

Supplier Data

Immunoprecipitation - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (AB289726)

This data was generated with ab289723, the same antibody in a different formulation.

c-Fos was immunoprecipitated from PC-12 whole cell lysate with ab289723 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab289723 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366), was used as secondary antibody at 1/5000 dilution.

Lane 1 : PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours whole cell lysate 10 μg (Input).

Lane 2 : ab289723 IP PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab289723 in PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours whole cell lysate.

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time : 8 seconds.

All lanes:

Immunoprecipitation - Anti-c-Fos antibody [EPR24046-20] (<a href='/en-us/products/primary-antibodies/c-fos-antibody-epr24046-20-ab289723'>ab289723</a>)

Predicted band size: 41 kDa

Observed band size: 55-62 kDa

false

Exposure time: 8s

• WB

Supplier Data

Western blot - Anti-c-Fos antibody [EPR24046-20] - BSA and Azide free (AB289726)

This data was developed using ab289723, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-c-Fos antibody [EPR24046-20] (<a href='/en-us/products/primary-antibodies/c-fos-antibody-epr24046-20-ab289723'>ab289723</a>) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma) starved overnight, whole cell lysate at 20 µg

Lane 2:

PC-12 starved overnight, then treated with 100ng/ml NGF and 10uM MG-132 for 2 hours, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 41 kDa

Observed band size: 55-62 kDa

false

Exposure time: 92s