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AB278642

Anti-c-Fos (phospho S32) antibody [cFosS32-BA9]

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(1 Publication)

Rabbit Recombinant Monoclonal c-Fos phospho S32 antibody. Suitable for Flow Cyt and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human FOS phospho S32.

View Alternative Names

G0S7, FOS, Protein c-Fos, Cellular oncogene fos, G0/G1 switch regulatory protein 7, Proto-oncogene c-Fos, Transcription factor AP-1 subunit c-Fos

4 Images
Flow Cytometry - Anti-c-Fos (phospho S32) antibody [cFosS32-BA9] (AB278642)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-c-Fos (phospho S32) antibody [cFosS32-BA9] (AB278642)

Flow cytometric analysis of HeLa cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV+TPA (green) using ab278642 at 0.001 µg/mL.

Flow Cytometry - Anti-c-Fos (phospho S32) antibody [cFosS32-BA9] (AB278642)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-c-Fos (phospho S32) antibody [cFosS32-BA9] (AB278642)

Peptide blocking flow cytometric analysis of HeLa cells secondary antibody only negative control (light blue) or untreated (red) or treated with UV + TPA (green) or untreated and blocked with phospho-peptide (black) or treated and blocked with phospho peptide (gold) or untreated and blocked with non-phospho peptide (dark blue) or treated and blocked with non-phospho peptide (purple) using ab278642 at 0.01µg/mL.

Flow Cytometry - Anti-c-Fos (phospho S32) antibody [cFosS32-BA9] (AB278642)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-c-Fos (phospho S32) antibody [cFosS32-BA9] (AB278642)

Flow cytometric analysis of C6 cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV+TPA (green) using ab278642 at 0.001 µg/mL.

Flow Cytometry - Anti-c-Fos (phospho S32) antibody [cFosS32-BA9] (AB278642)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-c-Fos (phospho S32) antibody [cFosS32-BA9] (AB278642)

Flow cytometric analysis of L929 cells, secondary antibody only negative control (blue) or untreated (red) or treated with UV (green) using ab278642 at 0.001 µg/mL.

  • 578 PE

    PE Anti-c-Fos (phospho S32) antibody [cFosS32-BA9]

  • 519 FITC

    FITC Anti-c-Fos (phospho S32) antibody [cFosS32-BA9]

  • 660 APC

    APC Anti-c-Fos (phospho S32) antibody [cFOSS32-BA9]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

cFosS32-BA9

Isotype

IgG

Light chain type

kappa

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt

applications

Immunogen

Synthetic Peptide within Human FOS phospho S32. The exact immunogen used to generate this antibody is proprietary information.

P01100

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49.88% PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The c-Fos protein also known as c-Fos is a component of the Fos family of transcription factors which includes FosB Fra-1 and Fra-2. These proteins play a role in regulating gene expression. c-Fos has a molecular weight of about 55 kDa. You usually find c-Fos expressed in various tissues including the brain providing a marker for neuronal activity and is often induced in response to stimuli like stress growth factors and mitogens. In research settings c-Fos staining and c-Fos immunohistochemistry are techniques commonly used to study activation patterns in cells and tissues.
Biological function summary

C-Fos acts as part of the Activator Protein-1 (AP-1) complex a group of DNA-binding proteins that regulate gene transcription. This complex forms when c-Fos and Jun proteins dimerize. The AP-1 complex controls several cellular processes such as proliferation differentiation and apoptosis. Due to its participation in these vital processes c-Fos influences cellular responses to external stimuli and can affect how cells behave under stress.

Pathways

C-Fos significantly contributes to the MAPK/ERK pathway which plays a role in cell growth and differentiation. It partners with Jun proteins to form functional transcription factors within the MAPK signaling cascade. Additionally c-Fos is involved in the JNK signaling pathway linking it to stress responses and apoptosis. These pathways highlight the partnerships between c-Fos and other proteins like ERK and JNK indicating their intertwined roles in cellular signaling networks.

Researchers associate c-Fos with cancer and neurodegenerative diseases. In cancer c-Fos expression correlates with changes in AP-1 activity which can lead to altered cell growth and proliferation potentially contributing to tumor formation. Its involvement in neurodegenerative diseases reveals its role in neuron apoptosis and cellular stress responses. In these scenarios proteins like Bcl-2 also play a part with CDK1 sharing connections in processes that affect cell cycle and apoptosis illustrating c-Fos's broad significance in both normal and pathological conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, FOS and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. On TGF-beta activation, forms a multimeric SMAD3/SMAD4/JUN/FOS complex at the AP1/SMAD-binding site to regulate TGF-beta-mediated signaling. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation. In growing cells, activates phospholipid synthesis, possibly by activating CDS1 and PI4K2A. This activity requires Tyr-dephosphorylation and association with the endoplasmic reticulum.
See full target information FOS phospho S32
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:15525-15534 PubMed38159254

2024

M-CSF secreted by gastric cancer cells exacerbates the progression of gastric cancer by increasing the expression of SHP2 in tumor-associated macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Shaohua Zhang,Dongfei Ren,Huiyu Hou,Li Yao,Hufang Yuan
View all publications
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